Translational control by secondary-structure formation in mRNA in a eukaryotic system.

Translational control by secondary-structure formation in mRNA in a eukaryotic system.
Nucleosides Nucleotides Nucleic Acids. 2019 Sep 30;:1-9
Authors: Kawaguchi D, Shimizu S, Abe N, Hashiya F, Tomoike F, Kimura Y, Abe H
Abstract
Eukaryotic mRNA has a cap structure at the 5' end and a poly(A) tail at the 3' end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.
PMID: 31566082 [PubMed - as supplied by publisher]

Source: Nucleosides, Nucleotides and Nucleic Acids - October 1, 2019 Category: Biochemistry Tags: Nucleosides Nucleotides Nucleic Acids Source Type: research