Construction of a genetically engineered strain of nattokinase and assessment of its fibrinolytic activity

A strain of Bacillus natto was isolated from natto and found to have a high yield of nattokinase. The aprN and pro-aprN gene fragments, encoding nattokinase from Bacillus natto, were amplified using two pair of primers and were expressed in Bacillus subtilis WB800N host cells using the pHT43 plasmid as a vector. In this system, the effect of leader peptides on nattokinase activity, revealing...
Source: African Journal of Microbiology Research - Category: Microbiology Source Type: research