Rv0100, a proposed acyl carrier protein in Mycobacterium tuberculosis: expression, purification and crystallization

Acyl carrier proteins (ACPs) are important components in fatty-acid biosynthesis in prokaryotes. Rv0100 is predicted to be an essential ACP in Mycobacterium tuberculosis, the pathogen that is the causative agent of tuberculosis, and therefore has the potential to be a novel antituberculosis drug target. Here, the successful cloning and purification of Rv0100 using Mycobacterium smegmatis as a host is reported. Crystals of the purified protein were obtained that diffracted to a resolution of 1.9   Å . Overall, this work lays the foundation for the future pursuit of drug discovery and development against this potentially novel drug target.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: acyl carrier protein Mycobacterium tuberculosis Mycobacterium smegmatis Rv0100 research communications Source Type: research

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Conclusion: HIV patients with the disseminated TB have higher risk for ocular TB. As ocular symptoms are rare, still all of them need a detailed ocular examination to look for active ocular TB which will reclassify isolated pulmonary/extrapulmonary to disseminated TB warranting a detailed systemic examination. PMID: 32083986 [PubMed - as supplied by publisher]
Source: Ocular Immunology and Inflammation - Category: Allergy & Immunology Tags: Ocul Immunol Inflamm Source Type: research
Authors: De Smet D, Payen MC, Remes J, Van den Wijngaert S, Vouche M, Konopnicki D PMID: 32081503 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research
Publication date: Available online 27 January 2020Source: Current Medicine Research and PracticeAuthor(s): K.K. Chopra, Shweta SinghAbstractTuberculosis (TB) has remained a disease of public health importance since ages, affecting more than 10 million people globally and taking lives of 2 million people worldwide every year. Despite the dramatic improvements made in providing high-quality TB diagnostic services, since the discovery of the causative bacilli, many people with TB remain undiagnosed or get diagnosed only after long delays. Ten countries account for 77% of this gap and use only smear microscopy for diagnosis, w...
Source: Current Medicine Research and Practice - Category: General Medicine Source Type: research
Publication date: Available online 21 February 2020Source: Microbes and InfectionAuthor(s): Patrick Howlett, Elsa Du Bruyn, Hazel Morrison, Isiguzo C. Godsent, Katalin A. Wilkinson, Mpiko Ntsekhe, Robert J. WilkinsonAbstractTuberculous pericarditis is a severe form of extrapulmonary tuberculosis and is the commonest cause of pericardial effusion in high incidence settings. Mortality ranges between 8-34%, and it is the leading cause of pericardial constriction in Africa and Asia. Current understanding of the disease is based on models derived from studies performed in the 1940-50s. This review summarises recent advances in ...
Source: Microbes and Infection - Category: Infectious Diseases Source Type: research
Publication date: April 2020Source: Cancer Epidemiology, Volume 65Author(s): Faezeh Salamat, Mahmoud Khandashpour, Mohammad Naeimi-Tabiei, Ali Ariannia, Mohammad Ashaari, SeyedMehdi Sedaghat, Fatemeh Ghasemi-Kebria, Fatemeh Salamat, Abbas Moghaddami, Susan Hasanpour-Heidari, Reza Hoseinpour, Nastaran Jafari-Delouei, Hamid Ghayoriardahaei, Masoomeh Gholami, Abdolreza Fazel, Gholamreza Roshandel, Jacques Ferlay, Elisabete WeiderpassAbstractIntroductionWe aimed to present the time trends and geographical distribution of lung cancer in Golestan province, a high-risk area for pulmonary tuberculosis (TB) in Northern Iran (2004-2...
Source: Cancer Epidemiology - Category: Cancer & Oncology Source Type: research
Publication date: 20 February 2020Source: Cell, Volume 180, Issue 4Author(s): Jonathan M. Stokes, Kevin Yang, Kyle Swanson, Wengong Jin, Andres Cubillos-Ruiz, Nina M. Donghia, Craig R. MacNair, Shawn French, Lindsey A. Carfrae, Zohar Bloom-Ackerman, Victoria M. Tran, Anush Chiappino-Pepe, Ahmed H. Badran, Ian W. Andrews, Emma J. Chory, George M. Church, Eric D. Brown, Tommi S. Jaakkola, Regina Barzilay, James J. CollinsSummaryDue to the rapid emergence of antibiotic-resistant bacteria, there is a growing need to discover new antibiotics. To address this challenge, we trained a deep neural network capable of predicting mole...
Source: Cell - Category: Cytology Source Type: research
ConclusionOur novel rabbit LAM-specific mAbs performed well at recognizing LAM from slow-growing pathogenic mycobacteria, supporting their future diagnostic potential.
Source: Journal of Microbiology, Immunology and Infection - Category: Microbiology Source Type: research
Multidrug resistant tuberculosis (MDR-TB) remains a serious public health problem with poor treatment outcomes. Predictors of poor outcomes vary in different regions. Vietnam is among the top 30 high burden of...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
It is estimated that one fourth of world population is infected with M. tuberculosis (Mtb) 1-5 and that only the 5-10 % of Mtb-infected individuals will progress to tuberculosis (TB) disease during life time.2,5 In 2017, 1 million (9%) of the worldwide TB cases were related to HIV-infected persons leading to 300,000 deaths.5 HIV-infection is responsible of an increased risk of progression to active disease, representing one of the main cause for active-TB disease, even in the absence of a deep CD4 T-cell impairment and even if under antiretroviral therapy (ART).
Source: Journal of Infection - Category: Infectious Diseases Authors: Tags: Original article Source Type: research
Today, interferon-gamma release assays (IGRAs) are essential tools for detecting infection with Mycobacterium tuberculosis (M. tuberculosis), including for diagnosing latent tuberculosis infection (LTBI). IGRAs, including both QuantiFERON (QFT) and T-SPOT.TB, enable direct observation of the response of a patient's blood cells to specific antigens derived from M. tuberculosis.1,2 On the other hand, IGRAs were not useful for accurately discriminating between active tuberculosis (active TB) and LTBI.
Source: Journal of Infection - Category: Infectious Diseases Authors: Source Type: research
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