Chapter Three - CryoEM reconstruction approaches to resolve asymmetric features

Publication date: 2019Source: Advances in Virus Research, Volume 105Author(s): Daniel J. Goetschius, Hyunwook Lee, Susan HafensteinAbstractAlthough icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.

Source: Advances in Virus Research - September 13, 2019 Category: Virology Source Type: research