Isolation of Human Peripheral Blood T-Lymphocytes.

Isolation of Human Peripheral Blood T-Lymphocytes.
Methods Mol Biol. 2019;1930:11-17
Authors: Kizhakeyil A, Ong ST, Fazil MHUT, Chalasani MLS, Prasannan P, Verma NK
Abstract
Peripheral blood is the most common source of T-lymphocytes for in vitro culture. Here, we present a simple and standardized method for small- or large-scale isolation of viable T-lymphocytes and other mononuclear cells from fresh peripheral blood or buffy coat blood samples using the density gradient centrifugation. T-cells obtained using the protocol described here can be used for a variety of downstream analysis, including cellular, molecular, and functional assays.
PMID: 30610593 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - August 21, 2019 Category: Molecular Biology Authors: Kizhakeyil A, Ong ST, Fazil MHUT, Chalasani MLS, Prasannan P, Verma NK Tags: Methods Mol Biol Source Type: research