LncRNA MALAT1 inhibits hypoxia/reoxygenationinduced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis.

LncRNA MALAT1 inhibits hypoxia/reoxygenationinduced human umbilical vein endothelial cell injury via targeting the microRNA-320a/RAC1 axis. Biol Chem. 2019 Aug 01;: Authors: Zhu R, Hu X, Xu W, Wu Z, Zhu Y, Ren Y, Cheng L Abstract Angiogenesis is believed to protect against hypoxia/reoxygenation (H/R)-induced cell injury. MALAT1 and microRNA-320a (miR-320a) are involved in cancer angiogenesis. To investigate the function of the MALAT1/miR-320a axis in H/R-induced cell injury, human umbilical vein endothelial cell (HUVEC) angiogenesis was detected using CCK-8, transwell migration, cell adhesion and tube formation assays. The expression of MALAT1 and miR- 320a was revealed by qRT-PCR. The direct binding relationship between miR-320a and MALAT1 was detected by RNA immunoprecipitation (RIP) and dual luciferase reporter assays. The data indicated that H/R induces angiogenesis injury and that the expression of MALAT1 was augmented in H/R-stimulated HUVECs. Overexpression of MALAT1 alleviated H/R-stimulated HUVEC dysfunction, whereas silencing of MALAT1 exerted the opposite effects. MALAT1 also reduced miR-320a levels in HUVECs. Overexpression of miR-320a repressed the function of MALAT1 on H/R-stimulated HUVECs, whereas inhibition of miR-320a exerted the opposite effect. Additionally, miR-320a inhibition alleviated H/R-stimulated HUVEC injury via RAC1. Taken together, this investigation concluded that MALAT1 represses H/R-stimulated HUVEC i...
Source: Biological Chemistry - Category: Chemistry Tags: Biol Chem Source Type: research