Comparison of the clinical signs, pathological and immuohistochemical findings in visceral organs of chickens experimentally infected with Salmonella Zega through three routes.

Comparison of the clinical signs, pathological and immuohistochemical findings in visceral organs of chickens experimentally infected with Salmonella Zega through three routes. Acta Trop. 2019 Aug 08;:105123 Authors: Mshelbwala FM, Ibrahim NDG, Saidu SN, Kadiri AKF, Kwanashie CN Abstract Salmonella Zega isolated from natural outbreaks that were characterized by high mortality in poultry farms in three Southwestern States of Nigeria was used to inoculate two week-old chicks through different routes in order to determine and compare the clinical signs, pathological and immunohistochemical changes in each route of infection. The birds were divided into 4 groups of 25 each as groups A (orally inoculated), B (intraperitoneally inoculated), C (inoculated per cloaca) and D (uninoculated control). All the birds were inoculated with 0.2 ml of 1 × 108 cfu of the bacteria. Clinical signs were observed and recorded according to the route of infection, and with the days post-infection from day 0 till day 10 post-infection. Two birds from each group were sacrificed every 24 hours and examined for gross lesions, which were described and scored according to the route of infection and days post-infection. Samples of visceral organs were collected for bacteriology, histopathology and immunohistochemistry. Clinical signs in chicks infected orally and intraperitoneally were weakness, anoraexia lethargy, somnolescence, yellowish diarrhoea observ...
Source: Acta Tropica - Category: Infectious Diseases Authors: Tags: Acta Trop Source Type: research