Simplified method for applying static isotropic tensile strain in cell culture experiments with identification of valid RT-qPCR reference genes for PDL fibroblasts.

CONCLUSIONS: A strain magnitude of 16 per cent for 48 hours elicited the most distinct cellular response by PDLF subjected to static tensile isotropic strain by the presented method. TBP and PPIB in conjunction proved to be the most appropriate reference genes to normalize target gene expression in RT-qPCR studies on PDLF subjected to tensile strain.
PMID: 31352484 [PubMed - as supplied by publisher]

Source: European Journal of Orthodontics - July 27, 2019 Category: Dentistry Authors: Nazet U, Schröder A, Spanier G, Wolf M, Proff P, Kirschneck C Tags: Eur J Orthod Source Type: research