Generation of CDMLe012-A-1 cells: A pluripotent human embryonic stem cell model of Turner's syndrome

Publication date: Available online 23 July 2019Source: Stem Cell ResearchAuthor(s): Aleksey Y. Domozhirov, John L. Mazzilli, Rick A. Wetsel, Eva M. ZsigmondAbstractMonosomy of chromosome X is associated with high prenatal mortality of female embryos and severe developmental abnormalities of patients born with Turner's syndrome (45,XO). The CDMLe012-A-1 human embryonic stem cell (hESC) line, derived from a day six blastocyst with a normal 46,XX female karyotype spontaneously lost an X-chromosome during cell culture. This 45,XO karyotype was stably maintained for more than 55 passages. Since the CDMLe012-A-1 cells express pluripotent stem cell markers and differentiate into cells derived from the three germ layers, the cell line represents a stable, pluripotent stem cell model of Turner's syndrome.
Source: Stem Cell Research - Category: Stem Cells Source Type: research