Methods for Analysis of Keratinocyte Migration.

Methods for Analysis of Keratinocyte Migration.
Methods Mol Biol. 2019 Jul 09;:
Authors: Liu J, Zhang J
Abstract
Reepithelialization of wounds involves several keratinocyte functions: proliferation, migration, and differentiation. The key step is keratinocyte migration, an essential aspect for understanding chronic non-healing wounds. Scratch wounding assay and cell motility assay are used to evaluate keratinocyte migration in vitro. The ability of keratinocyte migration is measured by the dynamic change of the wound closure area over time under a living-cell workstation. NIH ImageJ software is used to quantify the motility ability of single cell. The following protocols offer a general guideline for keratinocyte migration analysis.
PMID: 31286458 [PubMed - as supplied by publisher]

Source: Mol Biol Cell - July 8, 2019 Category: Molecular Biology Authors: Liu J, Zhang J Tags: Methods Mol Biol Source Type: research