Alkaline phosphatase activities and regulation in three harmful Prorocentrum species from the coastal waters of the East China Sea.

Alkaline phosphatase activities and regulation in three harmful Prorocentrum species from the coastal waters of the East China Sea. Microb Ecol. 2019 Jul 02;: Authors: Ou L, Qin X, Shi X, Feng Q, Zhang S, Lu S, Qi Y Abstract Harmful blooms of Prorocentrum donghaiense occur annually in the phosphorus-scarce coastal waters of the East China Sea (ECS). The enzymatic activities of alkaline phosphatase (AP) and its regulation by external phosphorus were studied during a P. donghaiense bloom in this area. The AP characteristics of P. donghaiense was further compared with Prorocentrum minimum and Prorocentrum micans in monocultures with both bulk and single-cell enzyme-labeled fluorescence AP assays. Concentrations of dissolved inorganic phosphorus (DIP) varied between 0.04 and 0.73 μmol l-1, with more than half recording stations registering concentrations below 0.10 μmol l-1. Concentrations of dissolved organic phosphorus (DOP) were comparable or even higher than those of DIP. P. donghaiense suffered phosphorus stress and expressed abundant AP, especially when DIP was lower than 0.10 μmol l-1. The AP activities showed a negative correlation with DIP but a positive correlation with DOP. The AP activities were also regulated by internal phosphorus pool. The sharp increase in AP activities was observed until cellular phosphorus was exhausted. Most AP of P. donghaiense was located on the cell surface and some were released into the water w...
Source: Microbial Ecology - Category: Microbiology Authors: Tags: Microb Ecol Source Type: research