Distinct Composition and Assembly Processes of Bacterial Communities in a River from the Arid Area: Ecotypes or Habitat Types?
In this study, the composition, potential function, and assembly mechanism of the bacterial community in the arid river were analysed. The Dirichlet multinomial mixture method recognised four ecotypes across the three habitats (biofilm, water, and sediment). The bacterial communities in water are more sensitive to human activities. Bacterial diversity and richness in water decreased as the intensity of human activities increased from the region of water II to water I. Significant differences in the composition and potential function profile of bacterial communities between water ecotypes were also observed, such as higher ...
Source: Microbial Ecology - October 23, 2021 Category: Microbiology Authors: Ran Qi Nana Xue Xiaobin Zhou Li Zhao Wenjuan Song Yuyi Yang Source Type: research

Microbial Community Shifts on Organic Rocks of Different Maturities Reveal potential Catabolisers of Organic Matter in Coal
This study seeks to expand those models by observing community compositional shifts in the presence of differing organic matter by conducting 16S rRNA microbial surveys using formation water from the Surat and Sydney Basins grown on varying types of organic matter (black and brown coal, oil shale, humic acid, and lignin). A total of 135 microbes were observed to become enriched in the presence of added organic matter in comparison to carbon-free treatments. These surveys allowed detailed analysis of microbial compositions in order to extrapolate which taxa favour growth in the presence of differing organic matter. This stu...
Source: Microbial Ecology - October 23, 2021 Category: Microbiology Authors: Andrew G McLeish Se Gong Paul Greenfield David J Midgley Ian T Paulsen Source Type: research

Transcriptional Changes in Bifidobacterium bifidum Involved in Synergistic Multispecies Biofilms
This study represents a comparative gene expression analysis of B. bifidum when grown in a single-species biofilm and in two multispecies biofilm consortia with Bifidobacterium longum subsp. infantis, Bacteroides ovatus, and Parabacteroides distasonis in order to identify genes involved in this adaptive process in mixed biofilms and the influence on its metabolic and functional traits. Changes up to 58% and 43% in its genome were found when it grew in three- and four-species biofilm consortia, respectively. Upregulation of genes of B. bifidum involved in carbohydrate metabolism (particularly the galE gene), quorum sensing ...
Source: Microbial Ecology - October 22, 2021 Category: Microbiology Authors: Faizan Ahmed Sadiq Lu Wenwei Chen Wei Zhao Jianxin Hao Zhang Source Type: research

Nutrient Removal in Eutrophic Water Promotes Stability of Planktonic Bacterial and Protist Communities
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01898-2. Online ahead of print.ABSTRACTNutrient (nitrogen and phosphorus) removal by using bioremediation technologies in eutrophic water alters bacterial and protist community structure and function, but how it changes the stability of community remains unclear. To fill this gap, in this study, bacterial and protist communities were investigated using 16S and 18S rRNA gene high-throughput sequencing during the nutrient removal by using ecological floating beds of Canna indica L. Our results showed that both bacterial and protist community compositions in the treatment grou...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Lemian Liu Shanshan Wang Jun Yang Jianfeng Chen Source Type: research

Habitat Disturbance Linked with Host Microbiome Dispersion and Bd Dynamics in Temperate Amphibians
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01897-3. Online ahead of print.ABSTRACTAnthropogenic habitat disturbances can dramatically alter ecological community interactions, including host-pathogen dynamics. Recent work has highlighted the potential for habitat disturbances to alter host-associated microbial communities, but the associations between anthropogenic disturbance, host microbiomes, and pathogens are unresolved. Amphibian skin microbial communities are particularly responsive to factors like temperature, physiochemistry, pathogen infection, and environmental microbial reservoirs. Through a field survey o...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Wesley J Neely Sasha E Greenspan Leigha M Stahl Sam D Heraghty Vanessa M Marshall Carla L Atkinson C Guilherme Becker Source Type: research

Microbial Diversity in Cultivated and Feral Vanilla Vanilla planifolia Orchids Affected by Stem and Rot Disease
We described the taxonomic composition of endophytic microbiomes in symptomatic and asymptomatic vanilla plants using 16S and ITS rDNA metabarcoding, and ITS Sanger sequences generated from fungal isolates. We compared the bacterial and fungal diversity in vanilla plants from a long-term plantation, and from feral plants found near abandoned plantations that did not present SRD symptoms. No significant differences were found in the species richness of the bacterial and fungal microbiome among feral, or asymptomatic and symptomatic cultivated vanilla. However, significant differences were detected in both fungal and bacteri...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Ireri A Carbajal-Valenzuela Ariel H Mu ñoz-Sanchez Juan Hern ández-Hernández Francisco Barona-G ómez Camille Truong Ang élica Cibrián-Jaramillo Source Type: research

Host Defense Metabolites Alter the Interactions between a Bark Beetle and its Symbiotic Fungi
Microb Ecol. 2021 Oct 21. doi: 10.1007/s00248-021-01894-6. Online ahead of print.ABSTRACTSuccessful host plant colonization by tree-killing bark beetle-symbiotic fungal complexes depends on host suitability, which is largely determined by host defense metabolites such as monoterpenes. Studies have shown the ability of specific blends of host monoterpenes to influence bark beetles or their fungal symbionts, but how biologically relevant blends of host monoterpenes influence bark beetle-symbiotic fungal interaction is unknown. We tested how interactions between two host species (lodgepole pine or jack pine) and two fungal sy...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Vanessa Agbulu Rashaduz Zaman Guncha Ishangulyyeva James F Cahill N Erbilgin Source Type: research

Nutrient Removal in Eutrophic Water Promotes Stability of Planktonic Bacterial and Protist Communities
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01898-2. Online ahead of print.ABSTRACTNutrient (nitrogen and phosphorus) removal by using bioremediation technologies in eutrophic water alters bacterial and protist community structure and function, but how it changes the stability of community remains unclear. To fill this gap, in this study, bacterial and protist communities were investigated using 16S and 18S rRNA gene high-throughput sequencing during the nutrient removal by using ecological floating beds of Canna indica L. Our results showed that both bacterial and protist community compositions in the treatment grou...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Lemian Liu Shanshan Wang Jun Yang Jianfeng Chen Source Type: research

Habitat Disturbance Linked with Host Microbiome Dispersion and Bd Dynamics in Temperate Amphibians
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01897-3. Online ahead of print.ABSTRACTAnthropogenic habitat disturbances can dramatically alter ecological community interactions, including host-pathogen dynamics. Recent work has highlighted the potential for habitat disturbances to alter host-associated microbial communities, but the associations between anthropogenic disturbance, host microbiomes, and pathogens are unresolved. Amphibian skin microbial communities are particularly responsive to factors like temperature, physiochemistry, pathogen infection, and environmental microbial reservoirs. Through a field survey o...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Wesley J Neely Sasha E Greenspan Leigha M Stahl Sam D Heraghty Vanessa M Marshall Carla L Atkinson C Guilherme Becker Source Type: research

Microbial Diversity in Cultivated and Feral Vanilla Vanilla planifolia Orchids Affected by Stem and Rot Disease
We described the taxonomic composition of endophytic microbiomes in symptomatic and asymptomatic vanilla plants using 16S and ITS rDNA metabarcoding, and ITS Sanger sequences generated from fungal isolates. We compared the bacterial and fungal diversity in vanilla plants from a long-term plantation, and from feral plants found near abandoned plantations that did not present SRD symptoms. No significant differences were found in the species richness of the bacterial and fungal microbiome among feral, or asymptomatic and symptomatic cultivated vanilla. However, significant differences were detected in both fungal and bacteri...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Ireri A Carbajal-Valenzuela Ariel H Mu ñoz-Sanchez Juan Hern ández-Hernández Francisco Barona-G ómez Camille Truong Ang élica Cibrián-Jaramillo Source Type: research

Nutrient Removal in Eutrophic Water Promotes Stability of Planktonic Bacterial and Protist Communities
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01898-2. Online ahead of print.ABSTRACTNutrient (nitrogen and phosphorus) removal by using bioremediation technologies in eutrophic water alters bacterial and protist community structure and function, but how it changes the stability of community remains unclear. To fill this gap, in this study, bacterial and protist communities were investigated using 16S and 18S rRNA gene high-throughput sequencing during the nutrient removal by using ecological floating beds of Canna indica L. Our results showed that both bacterial and protist community compositions in the treatment grou...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Lemian Liu Shanshan Wang Jun Yang Jianfeng Chen Source Type: research

Habitat Disturbance Linked with Host Microbiome Dispersion and Bd Dynamics in Temperate Amphibians
Microb Ecol. 2021 Oct 20. doi: 10.1007/s00248-021-01897-3. Online ahead of print.ABSTRACTAnthropogenic habitat disturbances can dramatically alter ecological community interactions, including host-pathogen dynamics. Recent work has highlighted the potential for habitat disturbances to alter host-associated microbial communities, but the associations between anthropogenic disturbance, host microbiomes, and pathogens are unresolved. Amphibian skin microbial communities are particularly responsive to factors like temperature, physiochemistry, pathogen infection, and environmental microbial reservoirs. Through a field survey o...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Wesley J Neely Sasha E Greenspan Leigha M Stahl Sam D Heraghty Vanessa M Marshall Carla L Atkinson C Guilherme Becker Source Type: research

Microbial Diversity in Cultivated and Feral Vanilla Vanilla planifolia Orchids Affected by Stem and Rot Disease
We described the taxonomic composition of endophytic microbiomes in symptomatic and asymptomatic vanilla plants using 16S and ITS rDNA metabarcoding, and ITS Sanger sequences generated from fungal isolates. We compared the bacterial and fungal diversity in vanilla plants from a long-term plantation, and from feral plants found near abandoned plantations that did not present SRD symptoms. No significant differences were found in the species richness of the bacterial and fungal microbiome among feral, or asymptomatic and symptomatic cultivated vanilla. However, significant differences were detected in both fungal and bacteri...
Source: Microbial Ecology - October 21, 2021 Category: Microbiology Authors: Ireri A Carbajal-Valenzuela Ariel H Mu ñoz-Sanchez Juan Hern ández-Hernández Francisco Barona-G ómez Camille Truong Ang élica Cibrián-Jaramillo Source Type: research

Ecological Dynamics and Co-occurrences Among Prokaryotes and Microeukaryotes in a Diatom Bloom Process in Xiangshan Bay, China
In this study, we investigated the ecological effects of an autumn diatom bloom on prokaryotic communities (PCCs) and microeukaryotic communities (MECs), focusing on their assembly processes and interactions. The PCCs were largely dominated by Alphaproteobacteria, Gammaproteobacteria, Cyanobacteria, and Flavobacteria, while the MECs primarily included Diatomea, Dinoflagellata, and Chlorophyta. The succession of both PCCs and MECs was mainly driven by this diatom bloom and environmental factors, such as nitrate and silicate. Null modeling revealed that homogeneous selection had a more pronounced impact on the structure of P...
Source: Microbial Ecology - October 19, 2021 Category: Microbiology Authors: Weijuan Xie Yi Yan Jian Hu Pengsheng Dong Dandi Hou Huajun Zhang Zhiyuan Yao Xiangyu Zhu Demin Zhang Source Type: research

Ecological Dynamics and Co-occurrences Among Prokaryotes and Microeukaryotes in a Diatom Bloom Process in Xiangshan Bay, China
In this study, we investigated the ecological effects of an autumn diatom bloom on prokaryotic communities (PCCs) and microeukaryotic communities (MECs), focusing on their assembly processes and interactions. The PCCs were largely dominated by Alphaproteobacteria, Gammaproteobacteria, Cyanobacteria, and Flavobacteria, while the MECs primarily included Diatomea, Dinoflagellata, and Chlorophyta. The succession of both PCCs and MECs was mainly driven by this diatom bloom and environmental factors, such as nitrate and silicate. Null modeling revealed that homogeneous selection had a more pronounced impact on the structure of P...
Source: Microbial Ecology - October 19, 2021 Category: Microbiology Authors: Weijuan Xie Yi Yan Jian Hu Pengsheng Dong Dandi Hou Huajun Zhang Zhiyuan Yao Xiangyu Zhu Demin Zhang Source Type: research

Ecological Dynamics and Co-occurrences Among Prokaryotes and Microeukaryotes in a Diatom Bloom Process in Xiangshan Bay, China
In this study, we investigated the ecological effects of an autumn diatom bloom on prokaryotic communities (PCCs) and microeukaryotic communities (MECs), focusing on their assembly processes and interactions. The PCCs were largely dominated by Alphaproteobacteria, Gammaproteobacteria, Cyanobacteria, and Flavobacteria, while the MECs primarily included Diatomea, Dinoflagellata, and Chlorophyta. The succession of both PCCs and MECs was mainly driven by this diatom bloom and environmental factors, such as nitrate and silicate. Null modeling revealed that homogeneous selection had a more pronounced impact on the structure of P...
Source: Microbial Ecology - October 19, 2021 Category: Microbiology Authors: Weijuan Xie Yi Yan Jian Hu Pengsheng Dong Dandi Hou Huajun Zhang Zhiyuan Yao Xiangyu Zhu Demin Zhang Source Type: research

Root-Associated Bacteria Community Characteristics of Antarctic Plants: Deschampsia antarctica and Colobanthus quitensis-a Comparison
In this study, we have explored and compared the rhizosphere and root-endosphere dwelling microbial community of C. quitensis and D. antarctica specimens sampled in maritime Antarctica from sites displaying contrasting edaphic characteristics. Bacterial phylogenetic diversity (high-throughput 16S rRNA gene fragment targeted sequencing) and microbial metabolic activity (Biolog EcoPlates) with a geochemical soil background were assessed. Gathered data showed that the microbiome of C. quitensis root system was mostly site-dependent, displaying different characteristics in each of the examined locations. This plant tolerated a...
Source: Microbial Ecology - October 18, 2021 Category: Microbiology Authors: Anna Zn ój Jan Gawor Robert Gromadka Katarzyna J Chwedorzewska Jakub Grzesiak Source Type: research

Himalayan Microbiomes for Agro-environmental Sustainability: Current Perspectives and Future Challenges
Microb Ecol. 2021 Oct 13. doi: 10.1007/s00248-021-01849-x. Online ahead of print.ABSTRACTThe Himalayas are one of the most mystical, yet least studied terrains of the world. One of Earth's greatest multifaceted and diverse montane ecosystems is also one of the thirty-four global biodiversity hotspots of the world. These are supposed to have been uplifted about 60-70 million years ago and support, distinct environments, physiography, a variety of orogeny, and great biological diversity (plants, animals, and microbes). Microbes are the pioneer colonizer of the Himalayas that are involved in various bio-geological cycles and ...
Source: Microbial Ecology - October 14, 2021 Category: Microbiology Authors: Deep Chandra Suyal Divya Joshi Saurabh Kumar Pankaj Bhatt Arun Narayan Krishna Giri Manali Singh Ravindra Soni Rakshak Kumar Ashok Yadav Rubee Devi Tanvir Kaur Divjot Kour Ajar Nath Yadav Source Type: research

Himalayan Microbiomes for Agro-environmental Sustainability: Current Perspectives and Future Challenges
Microb Ecol. 2021 Oct 13. doi: 10.1007/s00248-021-01849-x. Online ahead of print.ABSTRACTThe Himalayas are one of the most mystical, yet least studied terrains of the world. One of Earth's greatest multifaceted and diverse montane ecosystems is also one of the thirty-four global biodiversity hotspots of the world. These are supposed to have been uplifted about 60-70 million years ago and support, distinct environments, physiography, a variety of orogeny, and great biological diversity (plants, animals, and microbes). Microbes are the pioneer colonizer of the Himalayas that are involved in various bio-geological cycles and ...
Source: Microbial Ecology - October 14, 2021 Category: Microbiology Authors: Deep Chandra Suyal Divya Joshi Saurabh Kumar Pankaj Bhatt Arun Narayan Krishna Giri Manali Singh Ravindra Soni Rakshak Kumar Ashok Yadav Rubee Devi Tanvir Kaur Divjot Kour Ajar Nath Yadav Source Type: research

Himalayan Microbiomes for Agro-environmental Sustainability: Current Perspectives and Future Challenges
Microb Ecol. 2021 Oct 13. doi: 10.1007/s00248-021-01849-x. Online ahead of print.ABSTRACTThe Himalayas are one of the most mystical, yet least studied terrains of the world. One of Earth's greatest multifaceted and diverse montane ecosystems is also one of the thirty-four global biodiversity hotspots of the world. These are supposed to have been uplifted about 60-70 million years ago and support, distinct environments, physiography, a variety of orogeny, and great biological diversity (plants, animals, and microbes). Microbes are the pioneer colonizer of the Himalayas that are involved in various bio-geological cycles and ...
Source: Microbial Ecology - October 14, 2021 Category: Microbiology Authors: Deep Chandra Suyal Divya Joshi Saurabh Kumar Pankaj Bhatt Arun Narayan Krishna Giri Manali Singh Ravindra Soni Rakshak Kumar Ashok Yadav Rubee Devi Tanvir Kaur Divjot Kour Ajar Nath Yadav Source Type: research

Himalayan Microbiomes for Agro-environmental Sustainability: Current Perspectives and Future Challenges
Microb Ecol. 2021 Oct 13. doi: 10.1007/s00248-021-01849-x. Online ahead of print.ABSTRACTThe Himalayas are one of the most mystical, yet least studied terrains of the world. One of Earth's greatest multifaceted and diverse montane ecosystems is also one of the thirty-four global biodiversity hotspots of the world. These are supposed to have been uplifted about 60-70 million years ago and support, distinct environments, physiography, a variety of orogeny, and great biological diversity (plants, animals, and microbes). Microbes are the pioneer colonizer of the Himalayas that are involved in various bio-geological cycles and ...
Source: Microbial Ecology - October 14, 2021 Category: Microbiology Authors: Deep Chandra Suyal Divya Joshi Saurabh Kumar Pankaj Bhatt Arun Narayan Krishna Giri Manali Singh Ravindra Soni Rakshak Kumar Ashok Yadav Rubee Devi Tanvir Kaur Divjot Kour Ajar Nath Yadav Source Type: research

Ecological Processes and Human Behavior Provide a Framework for Studying the Skin Microbial Metacommunity
Microb Ecol. 2021 Oct 12. doi: 10.1007/s00248-021-01884-8. Online ahead of print.ABSTRACTMetacommunity theory dictates that a microbial community is supported both by local ecological processes and the dispersal of microbes between neighboring communities. Studies that apply this perspective to human-associated microbial communities are thus far limited to the gut microbiome. Yet, the skin serves as the primary barrier between the body and the external environment, suggesting frequent opportunities for microbial dispersal to the variable microbial communities that are housed across skin sites. This paper applies metacommun...
Source: Microbial Ecology - October 12, 2021 Category: Microbiology Authors: Melissa B Manus Source Type: research

Ecological Processes and Human Behavior Provide a Framework for Studying the Skin Microbial Metacommunity
Microb Ecol. 2021 Oct 12. doi: 10.1007/s00248-021-01884-8. Online ahead of print.ABSTRACTMetacommunity theory dictates that a microbial community is supported both by local ecological processes and the dispersal of microbes between neighboring communities. Studies that apply this perspective to human-associated microbial communities are thus far limited to the gut microbiome. Yet, the skin serves as the primary barrier between the body and the external environment, suggesting frequent opportunities for microbial dispersal to the variable microbial communities that are housed across skin sites. This paper applies metacommun...
Source: Microbial Ecology - October 12, 2021 Category: Microbiology Authors: Melissa B Manus Source Type: research

Dynamic Subspecies Population Structure of Vibrio cholerae in Dhaka, Bangladesh
Microb Ecol. 2021 Oct 11. doi: 10.1007/s00248-021-01838-0. Online ahead of print.ABSTRACTCholera has been endemic to the Ganges Delta for centuries. Although the causative agent, Vibrio cholerae, is autochthonous to coastal and brackish water, cholera occurs continually in Dhaka, the inland capital city of Bangladesh which is surrounded by fresh water. Despite the persistence of this problem, little is known about the environmental abundance and distribution of lineages of V. cholerae, the most important being the pandemic generating (PG) lineage consisting mostly of serogroup O1 strains. To understand spatial and temporal...
Source: Microbial Ecology - October 11, 2021 Category: Microbiology Authors: Tania Nasreen Mohammad Tarequl Islam Kevin Y H Liang Fatema-Tuz Johura Paul C Kirchberger Eric Hill Marzia Sultana Rebecca J Case Munirul Alam Yann F Boucher Source Type: research

Dynamic Subspecies Population Structure of Vibrio cholerae in Dhaka, Bangladesh
Microb Ecol. 2021 Oct 11. doi: 10.1007/s00248-021-01838-0. Online ahead of print.ABSTRACTCholera has been endemic to the Ganges Delta for centuries. Although the causative agent, Vibrio cholerae, is autochthonous to coastal and brackish water, cholera occurs continually in Dhaka, the inland capital city of Bangladesh which is surrounded by fresh water. Despite the persistence of this problem, little is known about the environmental abundance and distribution of lineages of V. cholerae, the most important being the pandemic generating (PG) lineage consisting mostly of serogroup O1 strains. To understand spatial and temporal...
Source: Microbial Ecology - October 11, 2021 Category: Microbiology Authors: Tania Nasreen Mohammad Tarequl Islam Kevin Y H Liang Fatema-Tuz Johura Paul C Kirchberger Eric Hill Marzia Sultana Rebecca J Case Munirul Alam Yann F Boucher Source Type: research

The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco
Microb Ecol. 2021 Oct 9. doi: 10.1007/s00248-021-01888-4. Online ahead of print.ABSTRACTIn this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains selected after REP-PCR fingerprinting showed that all the strains belong to the genus Bradyrhizobium. Following multi-locus sequence analysis (MLSA) using the rrs, gyrB, recA, glnII, and rpoB housek...
Source: Microbial Ecology - October 9, 2021 Category: Microbiology Authors: Omar Bouhnik Soufiane Alami Hanane Lamin Mouad Lamrabet Meryeme Bennis Mohammed Ouajdi Mhammed Bellaka Salwa El Antri Younes Abbas Hanaa Abdelmoumen Eulogio J Bedmar Mustapha Missbah El Idrissi Source Type: research

The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco
Microb Ecol. 2021 Oct 9. doi: 10.1007/s00248-021-01888-4. Online ahead of print.ABSTRACTIn this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains selected after REP-PCR fingerprinting showed that all the strains belong to the genus Bradyrhizobium. Following multi-locus sequence analysis (MLSA) using the rrs, gyrB, recA, glnII, and rpoB housek...
Source: Microbial Ecology - October 9, 2021 Category: Microbiology Authors: Omar Bouhnik Soufiane Alami Hanane Lamin Mouad Lamrabet Meryeme Bennis Mohammed Ouajdi Mhammed Bellaka Salwa El Antri Younes Abbas Hanaa Abdelmoumen Eulogio J Bedmar Mustapha Missbah El Idrissi Source Type: research

The Fodder Legume Chamaecytisus albidus Establishes Functional Symbiosis with Different Bradyrhizobial Symbiovars in Morocco
Microb Ecol. 2021 Oct 9. doi: 10.1007/s00248-021-01888-4. Online ahead of print.ABSTRACTIn this work, we analyzed the symbiotic performance and diversity of rhizobial strains isolated from the endemic shrubby legume Chamaecytisus albidus grown in soils of three different agroforestry ecosystems representing arid and semi-arid forest areas in Morocco. The analysis of the rrs gene sequences from twenty-four representative strains selected after REP-PCR fingerprinting showed that all the strains belong to the genus Bradyrhizobium. Following multi-locus sequence analysis (MLSA) using the rrs, gyrB, recA, glnII, and rpoB housek...
Source: Microbial Ecology - October 9, 2021 Category: Microbiology Authors: Omar Bouhnik Soufiane Alami Hanane Lamin Mouad Lamrabet Meryeme Bennis Mohammed Ouajdi Mhammed Bellaka Salwa El Antri Younes Abbas Hanaa Abdelmoumen Eulogio J Bedmar Mustapha Missbah El Idrissi Source Type: research

Short-Term Dynamics of Bdellovibrio and Like Organisms in Lake Geneva in Response to a Simulated Climatic Extreme Event
Microb Ecol. 2021 Oct 8. doi: 10.1007/s00248-021-01875-9. Online ahead of print.ABSTRACTThe short time-scale dynamics of three families of Bdellovibrio and like organisms (i.e. Bdellovibrionaceae, Peredibacteraceae, and Bacteriovoracaceae) were studied on the surface waters of Lake Geneva in summer. Using mesocosms deployed nearshore in July 2019, we simulated an extreme climatic event (an input of carbon from the watershed in response to runoff from the catchment, light reduction, and mixing in response to stormy conditions) and aimed to study the impact of both abiotic and biotic factors on their dynamics. The three fami...
Source: Microbial Ecology - October 8, 2021 Category: Microbiology Authors: J A Ezzedine A Janicot S Rasconi I Domaizon S Jacquet Source Type: research

Mosquito Microbiome Diversity Varies Along a Landscape-Scale Moisture Gradient
Microb Ecol. 2021 Oct 6. doi: 10.1007/s00248-021-01865-x. Online ahead of print.ABSTRACTMicroorganisms live in close association with metazoan hosts and form symbiotic microbiotas that modulate host biology. Although the function of host-associated microbiomes may change with composition, hosts within a population can exhibit high turnover in microbiome composition among individuals. However, environmental drivers of this variation are inadequately described. Here, we test the hypothesis that this diversity among the microbiomes of Aedes albopictus (a mosquito disease vector) is associated with the local climate and land-u...
Source: Microbial Ecology - October 7, 2021 Category: Microbiology Authors: Matthew C I Medeiros Priscilla S Seabourn Randi L Rollins Nicole M Yoneishi Source Type: research

Mosquito Microbiome Diversity Varies Along a Landscape-Scale Moisture Gradient
Microb Ecol. 2021 Oct 6. doi: 10.1007/s00248-021-01865-x. Online ahead of print.ABSTRACTMicroorganisms live in close association with metazoan hosts and form symbiotic microbiotas that modulate host biology. Although the function of host-associated microbiomes may change with composition, hosts within a population can exhibit high turnover in microbiome composition among individuals. However, environmental drivers of this variation are inadequately described. Here, we test the hypothesis that this diversity among the microbiomes of Aedes albopictus (a mosquito disease vector) is associated with the local climate and land-u...
Source: Microbial Ecology - October 7, 2021 Category: Microbiology Authors: Matthew C I Medeiros Priscilla S Seabourn Randi L Rollins Nicole M Yoneishi Source Type: research

Contrasting Responses of Arbuscular Mycorrhizal Fungal Families to Simulated Climate Warming and Drying in a Semiarid Shrubland
Microb Ecol. 2021 Oct 4. doi: 10.1007/s00248-021-01886-6. Online ahead of print.ABSTRACTWe carried out a 4-year manipulative field experiment in a semiarid shrubland in southeastern Spain to assess the impacts of experimental warming (W), rainfall reduction (RR), and their combination (W + RR) on the composition and diversity of arbuscular mycorrhizal fungal (AMF) communities in rhizosphere soil of H. syriacum and G. struthium shrubs using single-molecule real-time (SMRT) DNA sequencing. Across climate treatments, we encountered 109 AMF operational taxonomic units (OTUs) that were assigned to four families: Glomeraceae (93...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Mar ía Del Mar Alguacil Klaus Schlaeppi Álvaro López-García Marcel G A van der Heijden Jos é Ignacio Querejeta Source Type: research

In-Situ Biofloc Affects the Core Prokaryotes Community Composition in Gut and Enhances Growth of Nile Tilapia (Oreochromis niloticus)
In this study one recirculating aquaculture system was used to test how biofloc, including in-situ biofloc, dietary supplementation of ex-situ live or dead biofloc, influence fish gut prokaryotic community composition and growth performance in comparison to a biofloc-free control treatment. A core gut prokaryotic community was identified among all treatments by analyzing the temporal variations in gut prokaryotes. In-situ produced biofloc significantly increased the prokaryotic diversity in the gut by reducing the relative abundance of dominant Cetobacterium and increasing the relative abundance of potentially beneficial b...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Yale Deng Klaudyna Borewicz Joost van Loo Marko Zabala Olabarrieta Fotini Kokou Detmer Sipkema Marc C J Verdegem Source Type: research

Bee Trypanosomatids: First Steps in the Analysis of the Genetic Variation and Population Structure of Lotmaria passim, Crithidia bombi and Crithidia mellificae
Microb Ecol. 2021 Oct 5. doi: 10.1007/s00248-021-01882-w. Online ahead of print.ABSTRACTTrypanosomatids are among the most prevalent parasites in bees but, despite the fact that their impact on the colonies can be quite important and that their infectivity may potentially depend on their genotypes, little is known about the population diversity of these pathogens. Here we cloned and sequenced three non-repetitive single copy loci (DNA topoisomerase II, glyceraldehyde-3-phosphate dehydrogenase and RNA polymerase II large subunit, RPB1) to produce new genetic data from Crithidia bombi, C. mellificae and Lotmaria passim isola...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Carolina Bartolom é Mar ía Buendía-Abad Concepci ón Ornosa Pilar De la R úa Raquel Mart ín-Hernández Mariano Higes Xulio Maside Source Type: research

Contrasting Responses of Arbuscular Mycorrhizal Fungal Families to Simulated Climate Warming and Drying in a Semiarid Shrubland
Microb Ecol. 2021 Oct 4. doi: 10.1007/s00248-021-01886-6. Online ahead of print.ABSTRACTWe carried out a 4-year manipulative field experiment in a semiarid shrubland in southeastern Spain to assess the impacts of experimental warming (W), rainfall reduction (RR), and their combination (W + RR) on the composition and diversity of arbuscular mycorrhizal fungal (AMF) communities in rhizosphere soil of H. syriacum and G. struthium shrubs using single-molecule real-time (SMRT) DNA sequencing. Across climate treatments, we encountered 109 AMF operational taxonomic units (OTUs) that were assigned to four families: Glomeraceae (93...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Mar ía Del Mar Alguacil Klaus Schlaeppi Álvaro López-García Marcel G A van der Heijden Jos é Ignacio Querejeta Source Type: research

In-Situ Biofloc Affects the Core Prokaryotes Community Composition in Gut and Enhances Growth of Nile Tilapia (Oreochromis niloticus)
In this study one recirculating aquaculture system was used to test how biofloc, including in-situ biofloc, dietary supplementation of ex-situ live or dead biofloc, influence fish gut prokaryotic community composition and growth performance in comparison to a biofloc-free control treatment. A core gut prokaryotic community was identified among all treatments by analyzing the temporal variations in gut prokaryotes. In-situ produced biofloc significantly increased the prokaryotic diversity in the gut by reducing the relative abundance of dominant Cetobacterium and increasing the relative abundance of potentially beneficial b...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Yale Deng Klaudyna Borewicz Joost van Loo Marko Zabala Olabarrieta Fotini Kokou Detmer Sipkema Marc C J Verdegem Source Type: research

Bee Trypanosomatids: First Steps in the Analysis of the Genetic Variation and Population Structure of Lotmaria passim, Crithidia bombi and Crithidia mellificae
Microb Ecol. 2021 Oct 5. doi: 10.1007/s00248-021-01882-w. Online ahead of print.ABSTRACTTrypanosomatids are among the most prevalent parasites in bees but, despite the fact that their impact on the colonies can be quite important and that their infectivity may potentially depend on their genotypes, little is known about the population diversity of these pathogens. Here we cloned and sequenced three non-repetitive single copy loci (DNA topoisomerase II, glyceraldehyde-3-phosphate dehydrogenase and RNA polymerase II large subunit, RPB1) to produce new genetic data from Crithidia bombi, C. mellificae and Lotmaria passim isola...
Source: Microbial Ecology - October 5, 2021 Category: Microbiology Authors: Carolina Bartolom é Mar ía Buendía-Abad Concepci ón Ornosa Pilar De la R úa Raquel Mart ín-Hernández Mariano Higes Xulio Maside Source Type: research

Diversity of Microbial Functional Genes Should Be Considered During the Interpretation of the qPCR Melting Curves
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01883-9. Online ahead of print.ABSTRACTSoil microorganisms play an essential role in biogeochemical cycles. One approach to study these microbial communities is quantifying functional genes by quantitative PCR (qPCR), in which a melting curve analysis is usually assessed to confirm that a single PCR product is being quantified. However, the high diversity of functional genes in environmental samples could generate more than one peak in those curves, so the presence of two or multiple peaks does not always indicate nonspecific amplification. Here, we analyzed the taxonomic di...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Katerin Almendras Stefania Iannuzzi Margarita Car ú Julieta Orlando Source Type: research

Elevational Changes in Bacterial Microbiota Structure and Diversity in an Arthropod-Disease Vector
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01879-5. Online ahead of print.ABSTRACTEnvironmental conditions change rapidly along elevational gradients and have been found to affect community composition in macroscopic taxa, with lower diversity typically observed at higher elevations. In contrast, microbial community responses to elevation are still poorly understood. Specifically, the effects of elevation on vector-associated microbiota have not been studied to date, even though the within-vector microbial community is known to influence vector competence for a range of zoonotic pathogens. Here we characterize the st...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Tuomas Aivelo M élissa Lemoine Barbara Tschirren Source Type: research

Diversity of Microbial Functional Genes Should Be Considered During the Interpretation of the qPCR Melting Curves
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01883-9. Online ahead of print.ABSTRACTSoil microorganisms play an essential role in biogeochemical cycles. One approach to study these microbial communities is quantifying functional genes by quantitative PCR (qPCR), in which a melting curve analysis is usually assessed to confirm that a single PCR product is being quantified. However, the high diversity of functional genes in environmental samples could generate more than one peak in those curves, so the presence of two or multiple peaks does not always indicate nonspecific amplification. Here, we analyzed the taxonomic di...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Katerin Almendras Stefania Iannuzzi Margarita Car ú Julieta Orlando Source Type: research

Elevational Changes in Bacterial Microbiota Structure and Diversity in an Arthropod-Disease Vector
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01879-5. Online ahead of print.ABSTRACTEnvironmental conditions change rapidly along elevational gradients and have been found to affect community composition in macroscopic taxa, with lower diversity typically observed at higher elevations. In contrast, microbial community responses to elevation are still poorly understood. Specifically, the effects of elevation on vector-associated microbiota have not been studied to date, even though the within-vector microbial community is known to influence vector competence for a range of zoonotic pathogens. Here we characterize the st...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Tuomas Aivelo M élissa Lemoine Barbara Tschirren Source Type: research

Diversity of Microbial Functional Genes Should Be Considered During the Interpretation of the qPCR Melting Curves
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01883-9. Online ahead of print.ABSTRACTSoil microorganisms play an essential role in biogeochemical cycles. One approach to study these microbial communities is quantifying functional genes by quantitative PCR (qPCR), in which a melting curve analysis is usually assessed to confirm that a single PCR product is being quantified. However, the high diversity of functional genes in environmental samples could generate more than one peak in those curves, so the presence of two or multiple peaks does not always indicate nonspecific amplification. Here, we analyzed the taxonomic di...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Katerin Almendras Stefania Iannuzzi Margarita Car ú Julieta Orlando Source Type: research

Elevational Changes in Bacterial Microbiota Structure and Diversity in an Arthropod-Disease Vector
Microb Ecol. 2021 Oct 2. doi: 10.1007/s00248-021-01879-5. Online ahead of print.ABSTRACTEnvironmental conditions change rapidly along elevational gradients and have been found to affect community composition in macroscopic taxa, with lower diversity typically observed at higher elevations. In contrast, microbial community responses to elevation are still poorly understood. Specifically, the effects of elevation on vector-associated microbiota have not been studied to date, even though the within-vector microbial community is known to influence vector competence for a range of zoonotic pathogens. Here we characterize the st...
Source: Microbial Ecology - October 2, 2021 Category: Microbiology Authors: Tuomas Aivelo M élissa Lemoine Barbara Tschirren Source Type: research

Instability and Stasis Among the Microbiome of Seagrass Leaves, Roots and Rhizomes, and Nearby Sediments Within a Natural pH Gradient
The objective of the present study was to characterize the bacterial community of the leaves, bulk samples of roots and rhizomes, and proximal sediment of the seagrass species Cymodocea nodosa along the natural pH gradient of Levante Bay, Vulcano Island, Italy. The bacterial community was determined by characterizing the 16S rRNA amplicon sequencing and analyzing the operational taxonomic unit classification of bacterial DNA within samples. Statistical analyses were used to explore how life-long exposure to different pH/pCO2 conditions may be associated with significant differences in microbial communities, dominant bacter...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Raymond B Banister Melbert T Schwarz Maoz Fine Kim B Ritchie Erinn M Muller Source Type: research

Massoia Lactone Displays Strong Antifungal Property Against Many Crop Pathogens and Its Potential Application
Microb Ecol. 2021 Oct 1. doi: 10.1007/s00248-021-01885-7. Online ahead of print.ABSTRACTMassoia lactone could be released from liamocins produced by Aureobasidium melanogenum M39. The obtained Massoia lactone was very stable and highly active against many fungal crop pathogens which cause many plant diseases and food unsafety. Massoia lactone treatment not only could effectively inhibit their hyphal growth and spore germination, but also caused pore formation in cell membrane, reduction of ergosterol content, rise in intracellular ROS levels, and leakage of intracellular components, consequently leading to cellular necrosi...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Mei Zhang Zhi-Chao Gao Zhe Chi Zhu Wang Guang-Lei Liu Xue-Feng Li Zhong Hu Zhen-Ming Chi Source Type: research

Elevated Temperature May Affect Nectar Microbes, Nectar Sugars, and Bumble Bee Foraging Preference
Microb Ecol. 2021 Oct 1. doi: 10.1007/s00248-021-01881-x. Online ahead of print.ABSTRACTFloral nectar, an important resource for pollinators, is inhabited by microbes such as yeasts and bacteria, which have been shown to influence pollinator preference. Dynamic and complex plant-pollinator-microbe interactions are likely to be affected by a rapidly changing climate, as each player has their own optimal growth temperatures and phenological responses to environmental triggers, such as temperature. To understand how warming due to climate change is influencing nectar microbial communities, we incubated a natural nectar microb...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Kaleigh A Russell Quinn S McFrederick Source Type: research

Instability and Stasis Among the Microbiome of Seagrass Leaves, Roots and Rhizomes, and Nearby Sediments Within a Natural pH Gradient
The objective of the present study was to characterize the bacterial community of the leaves, bulk samples of roots and rhizomes, and proximal sediment of the seagrass species Cymodocea nodosa along the natural pH gradient of Levante Bay, Vulcano Island, Italy. The bacterial community was determined by characterizing the 16S rRNA amplicon sequencing and analyzing the operational taxonomic unit classification of bacterial DNA within samples. Statistical analyses were used to explore how life-long exposure to different pH/pCO2 conditions may be associated with significant differences in microbial communities, dominant bacter...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Raymond B Banister Melbert T Schwarz Maoz Fine Kim B Ritchie Erinn M Muller Source Type: research

Massoia Lactone Displays Strong Antifungal Property Against Many Crop Pathogens and Its Potential Application
Microb Ecol. 2021 Oct 1. doi: 10.1007/s00248-021-01885-7. Online ahead of print.ABSTRACTMassoia lactone could be released from liamocins produced by Aureobasidium melanogenum M39. The obtained Massoia lactone was very stable and highly active against many fungal crop pathogens which cause many plant diseases and food unsafety. Massoia lactone treatment not only could effectively inhibit their hyphal growth and spore germination, but also caused pore formation in cell membrane, reduction of ergosterol content, rise in intracellular ROS levels, and leakage of intracellular components, consequently leading to cellular necrosi...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Mei Zhang Zhi-Chao Gao Zhe Chi Zhu Wang Guang-Lei Liu Xue-Feng Li Zhong Hu Zhen-Ming Chi Source Type: research

Elevated Temperature May Affect Nectar Microbes, Nectar Sugars, and Bumble Bee Foraging Preference
Microb Ecol. 2021 Oct 1. doi: 10.1007/s00248-021-01881-x. Online ahead of print.ABSTRACTFloral nectar, an important resource for pollinators, is inhabited by microbes such as yeasts and bacteria, which have been shown to influence pollinator preference. Dynamic and complex plant-pollinator-microbe interactions are likely to be affected by a rapidly changing climate, as each player has their own optimal growth temperatures and phenological responses to environmental triggers, such as temperature. To understand how warming due to climate change is influencing nectar microbial communities, we incubated a natural nectar microb...
Source: Microbial Ecology - October 1, 2021 Category: Microbiology Authors: Kaleigh A Russell Quinn S McFrederick Source Type: research

Metagenomic Analysis of Biochemical Passive Reactors During Acid Mine Drainage Bioremediation Reveals Key Co-selected Metabolic Functions
Microb Ecol. 2021 Sep 30. doi: 10.1007/s00248-021-01872-y. Online ahead of print.ABSTRACTAcid mine drainage (AMD) is the major pollutant generated by the mining industry, and it is characterized by low pH and high concentration of metals and sulfate. The use of biochemical passive reactors (BPRs) is a promising strategy for its bioremediation. To date, there are various studies describing the taxonomical composition of BPR microbial communities, generally consisting of an assemblage of sulfate-reducing organisms inside Deltaproteobacteria, and a diverse set of anaerobic (ligno)cellulolytic bacteria; however, insights about...
Source: Microbial Ecology - September 30, 2021 Category: Microbiology Authors: Marcela Villegas-Plazas Janeth Sanabria Ziv Arbeli Yaneth Vasquez Fabio Roldan Howard Junca Source Type: research