The Pathometabolism of Legionella Studied by Isotopologue Profiling.

The Pathometabolism of Legionella Studied by Isotopologue Profiling.
Methods Mol Biol. 2019;1921:21-44
Authors: Heuner K, Kunze M, Chen F, Eisenreich W
Abstract
Metabolic pathways and fluxes can be analyzed under in vivo conditions by incorporation experiments using general 13C-labelled precursors. On the basis of the isotopologue compositions in amino acids or other metabolites, the incorporation rates of the supplied precursors and the pathways of their utilization can be studied in considerable detail. In this chapter, the method of isotopologue profiling is illustrated with recent work on the metabolism of intracellular living Legionella pneumophila.
PMID: 30694483 [PubMed - indexed for MEDLINE]

Source: Mol Biol Cell - June 22, 2019 Category: Molecular Biology Authors: Heuner K, Kunze M, Chen F, Eisenreich W Tags: Methods Mol Biol Source Type: research