Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.

Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos. Methods Mol Biol. 2019;1920:143-162 Authors: Buckley CE Abstract This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an "anchor" protein and a "bait" protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytochrome heterodimerization system in early zebrafish embryos (Buckley et al., Dev Cell 36(1):117-126, 2016) and describe the imaging parameters used to achieve subcellular light patterning. PMID: 30737691 [PubMed - indexed for MEDLINE]
Source: Mol Biol Cell - Category: Molecular Biology Authors: Tags: Methods Mol Biol Source Type: research