Conformational plasticity of the intracellular cavity of GPCR-G-protein complexes leads to G-protein promiscuity and selectivity [Medical Sciences]

While the dynamics of the intracellular surface in agonist-stimulated GPCRs is well studied, the impact of GPCR dynamics on G-protein selectivity remains unclear. Here, we combine molecular dynamics simulations with live-cell FRET and secondary messenger measurements, for 21 GPCR−G-protein combinations, to advance a dynamic model of the GPCR−G-protein interface. Our...

Source: Proceedings of the National Academy of Sciences - June 10, 2019 Category: Science Authors: Manbir Sandhu, Anja M. Touma, Matthew Dysthe, Fredrik Sadler, Sivaraj Sivaramakrishnan, Nagarajan Vaidehi Tags: PNAS Plus Source Type: research