RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions.

RAB1A promotes Vaccinia virus replication by facilitating the production of intracellular enveloped virions.
Virology. 2014 Nov 25;475C:66-73
Authors: Pechenick Jowers T, Featherstone RJ, Reynolds DK, Brown HK, James J, Prescott A, Haga IR, Beard PM
Abstract
Vaccinia virus (VACV) is a large double-stranded DNA virus with a complex cytoplasmic replication cycle that exploits numerous cellular proteins. This work characterises the role of a proviral cellular protein, the small GTPase RAB1A, in VACV replication. Using siRNA, we identified RAB1A as required for the production of extracellular enveloped virions (EEVs), but not intracellular mature virions (IMVs). Immunofluorescence and electron microscopy further refined the role of RAB1A as facilitating the wrapping of IMVs to become intracellular enveloped virions (IEVs). This is consistent with the known function of RAB1A in maintenance of ER to Golgi transport. VACV can therefore be added to the growing list of viruses which require RAB1A for optimal replication, highlighting this protein as a broadly proviral host factor.
PMID: 25462347 [PubMed - as supplied by publisher]

Source: Virology - November 25, 2014 Category: Virology Authors: Pechenick Jowers T, Featherstone RJ, Reynolds DK, Brown HK, James J, Prescott A, Haga IR, Beard PM Tags: Virology Source Type: research