Soy hydrolysate mimic autocrine growth factors effect of conditioned media to promote single CHO-K1 cell proliferation.

Soy hydrolysate mimic autocrine growth factors effect of conditioned media to promote single CHO-K1 cell proliferation.
Tissue Cell. 2019 Jun;58:130-133
Authors: Xu W, Yu X, Zhang J, Bhushan S, Prasad S, Prasad KN, Wu F, Yuan J, Poon HF
Abstract
The increasingly competitive biopharmaceutical industry requires companies to focus on rapid and low-cost cell line development. Single-cell cloning (SCC) is a critical and high-value process for cell line development, and typically problematic because single cell proliferates slowly when cultivated at low cell densities. Conditioned media (CM) provide autocrine growth factors to facilitate single cell proliferation, thus improve SCC efficiency. However, conditioned media (CM) are not a feasible solution for industrial cell line development due to variation and cross-contamination concerns. Here, we have found an improvement in the SCC efficiency similar to CM when soy hydrolysate was supplemented in SCC media. Therefore, we concluded that hydrolysate can mimic the autocrine growth factor(s) effect to improve cloning efficiency observed in CM.
PMID: 31133241 [PubMed - in process]

Source: Tissue and Cell - May 29, 2019 Category: Cytology Authors: Xu W, Yu X, Zhang J, Bhushan S, Prasad S, Prasad KN, Wu F, Yuan J, Poon HF Tags: Tissue Cell Source Type: research