Multifunctional motion-to-color janus transducers for the rapid detection of sepsis biomarkers in whole blood

Publication date: Available online 25 May 2019Source: Biosensors and BioelectronicsAuthor(s): Steven M. Russell, Alejandra Alba-Patiño, Marcio Borges, Roberto de la RicaAbstractSelf-propelled particles are revolutionizing sensing applications thanks to a unique motion-based signal generation mechanism in which biorecognition reactions are detected as changes in the velocity of the colloids. Here a new family of self-propelled multifunctional Janus particles is introduced that enables detecting changes in particle motion colorimetrically. The particles consist of an iron oxide core that provides color and magnetism, and a Janus coating that provides biospecific recognition and locomotive properties. In this approach, biomolecular interactions trigger changes in particle motion that are detected as variations in color when spotted on a piece of paper. These variations in color are then read and quantified with a custom-made smartphone app. The high surface area and magnetism of the particles makes them ideal building blocks for developing biosensors because they allow for the rapid capture of a target molecule and the removal of non-specific interactions. Biosensors engineered with the proposed multifunctional particles were able to detect the sepsis biomarker procalcitonin at clinically relevant concentrations within 13 min in whole blood, which is faster than other approaches requiring hour-long incubation steps under controlled conditions to detect the same biomarker in p...

Source: Biosensors and Bioelectronics - May 25, 2019 Category: Biotechnology Source Type: research