A new chromatographic approach to analyze methylproteome with enhanced lysine methylation identification performance.

In this study, we presented a novel chromatography-based enrichment method for global methylproteome analysis. The offline multidimensional tandem chromatography combining strong cation exchange (SCX) chromatography, immobilized metal ion affinity chromatography (IMAC) and high-pH reversed-phase chromatography (high-pH RP) was applied in the large-scale analysis of methylproteome. Totally, 860 forms on 765 sites were identified from BEL cells, covering all five arginine/lysine methylation forms. Among them, 27.21% were lysine methylation forms. This technique allows the simultaneous analysis of both arginine and lysine methylation while it has improved performance for the identification of lysine methylation. Therefore, it is a promising strategy for the investigation of biological functions related to methylation.
PMID: 31072472 [PubMed - in process]

Source: Analytica Chimica Acta - May 11, 2019 Category: Chemistry Authors: Wang Q, Liu Z, Wang K, Wang Y, Ye M Tags: Anal Chim Acta Source Type: research