High-sensitive immunosensing of protein biomarker based on interfacial recognition-induced homogeneous exponential transcription.

High-sensitive immunosensing of protein biomarker based on interfacial recognition-induced homogeneous exponential transcription.
Anal Chim Acta. 2019 Aug 27;1067:107-114
Authors: Teng J, Huang L, Zhang L, Li J, Bai H, Li Y, Ding S, Zhang Y, Cheng W
Abstract
A novel and versatile immunosensing strategy was developed for ultrasensitive and specific detection of proteins by organically integrating interfacial specific target recognition and homogeneous transcription amplification. In principle, classic antigen-antibody sandwich structure on the microplate could realize the specific identification of target protein. Biotinylated DNA probe was subsequently introduced by streptavidin-biotin system as a bridge linking interfacial and homogeneous reaction. The biotinylated DNA initiated exponential transcription amplification in the solution, which converted per target recognition event on the interface to numerous single-stranded RNA products in solution for highly sensitive fluorescence immunosensing. The proposed immunoassay based on interfacial recognition-induced homogeneous exponential transcription (IR-HET) for vascular endothelial growth factor (VEGF) detection showed a good linear range from 0.01 to 1000 pg/mL and the limit of detection as low as 1 fg/mL, which was 3 orders lower than traditional ELISA method. The established strategy was also successfully applied to directly detect VEGF from culture supernatants of tumor cells...

Source: Analytica Chimica Acta - May 4, 2019 Category: Chemistry Authors: Teng J, Huang L, Zhang L, Li J, Bai H, Li Y, Ding S, Zhang Y, Cheng W Tags: Anal Chim Acta Source Type: research