Caveolin-1 Scaffolding Domain Peptide Prevents Hyperoxia-Induced Airway Remodeling in a Neonatal Mouse Model.

Caveolin-1 Scaffolding Domain Peptide Prevents Hyperoxia-Induced Airway Remodeling in a Neonatal Mouse Model. Am J Physiol Lung Cell Mol Physiol. 2019 May 01;: Authors: Vogel ER, Manlove LJ, Kuipers I, Thompson MA, Fang YH, Freeman MR, Britt RD, Faksh A, Yang B, Prakash YS, Pabelick CM Abstract Reactive airway diseases are significant sources of pulmonary morbidity in neonatal and pediatric patients. Supplemental oxygen exposure in premature infants contributes to airway diseases such as asthma, and promotes development of airway remodeling, characterized by increased airway smooth muscle (ASM) mass and extracellular matrix (ECM) deposition. Decreased plasma membrane caveolin-1 (CAV1) expression has been implicated in airway disease and may contribute to airway remodeling and hyperreactivity. Here, we investigated the impact of clinically relevant moderate hyperoxia (50% O2) on airway remodeling and caveolar protein expression in a neonatal mouse model. Within 12 hours of birth, litters of B6129SF2J mice were randomized to room air (RA) or 50% hyperoxia exposure for 7 days with or without Caveolin-1 scaffolding domain peptide (CSD; caveolin-1 mimic; 10 µL, 0.25 mM daily via intraperitoneal (IP) injection) followed by 14 days recovery in normoxia Moderate hyperoxia significantly increased airway reactivity and decreased pulmonary compliance at 3 weeks. Histologic assessment demonstrated airway wall thickening, and increased ASM mass ...
Source: Am J Physiol Lung Ce... - Category: Respiratory Medicine Authors: Tags: Am J Physiol Lung Cell Mol Physiol Source Type: research