MicroRNA-31 Negatively Regulates Interleukin-34 Expression In Vitro.

MicroRNA-31 Negatively Regulates Interleukin-34 Expression In Vitro. Immunol Invest. 2019 Apr 23;:1-11 Authors: Li M, Dong Y, Chen Z, Meng L, Liu X, Zhang X, Wang H, Mao W, Zhang J, Jiang Z, Huang T, Hu J, Luo P, Korner H, Ying S, Li J Abstract Interleukin-34 (IL-34) is a recently discovered cytokine that promotes tissue macrophage maturation and differentiation. We previously found that 1α,25-Dihydroxyvitamin D3 up-regulated IL-34 expression in SH-SY5Y neural cells. However, whether microRNA regulates IL-34 expression is not completely clear. By using on-line TargetScan and MiRanda software, we found that there was only one conserved microRNA-31 (miR-31) binding site in the 3' untranslated region (3'UTR) of IL-34 mRNA. Intriguingly, using qPCR we demonstrated that miR-31 levels were negatively correlated to IL-34 mRNA levels in different cell lines. By examining the effect of miR-31 on IL-34 3' UTR reporter luciferase activity and on IL-34 mRNA and argonaute RISC catalytic component 2 (AGO2) binding, it was found that miR-31 bound directly to IL-34 3'UTR and regulated the post-transcriptional expression of IL-34 in MGC-803 cells. Moreover, a miR-31 mimic significantly reduced IL-34 expression levels while a miR-31 inhibitor up-regulated IL-34 expression in KYSE-45 and HT-29 cells. Taken together, these results show that miR-31 negatively regulates IL-34 expression by directly binding to the IL-34 3' UTR in vitro. PMID: 3101...
Source: Immunological Investigations - Category: Allergy & Immunology Tags: Immunol Invest Source Type: research