Two/three-dimensional interfacial properties of the novel peptide as a selective destroyer of biomembrane

In this study, a novel amphiphilic peptide (amPE) was designed for antimicrobial agent. Its thermal responsiveness in solution and in liposome membrane was studied by circular dichroism (CD). Then, amPE’s adsorption and further insertion behaviors at lipid monolayer were investigated by Langmuir-Blodgett (LB) and polarization modulation infrared reflection adsorption spectroscopy (PM-IRRAS). Results showed that amPE could selectively insert into DPPG monolayer due to the electrostatic interaction and cause an increase in content of α-helix. Adsorption kinetics under different initial surface pressures showed higher surface pressure prevented peptide’s insertion. Then, experiments of dye leakage from liposomes were carried for discussing how amPE disturbed the liposome membrane. Serious dye leakage from DPPG liposome was observed and the leakage was further improved when temperature increased from 37 °C to 45 °C. The designed amPE showed its good potential as antimicrobial/antitumor agent and great superiority of thermal responsiveness.Graphical abstractThe designed amPE inserted into biomembrane and induced greater dye leakage for its thermal responsiveness.

Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - April 22, 2019 Category: Chemistry Source Type: research