017 Immunization of dermatomyositis-specific autoantigen transcriptional intermediary factor (TIF1)- γ induces experimental myositis in mice

A number of myositis-specific autoantigens have been identified in dermatomyositis patients. While murine models of experimental myositis have been established using immunizations of muscle-specific proteins like myosin and C protein, they are not targeted in actual diseases. Here we established a new murine model of experimental myositis inducible with immunizations of TIF1- γ, one of self-antigens for myositis-specific autoantibodies. We purified recombinant whole human TIF1-γ protein using baculovirus expression systems, and immunized wild-type C57BL/6J (B6) mice with subcutaneous injections of emulsions containing the protein and complete Freund's adjuvant four tim es.
Source: Journal of Investigative Dermatology - Category: Dermatology Authors: Tags: Adaptive and Auto-Immunity Source Type: research