A ratiometric fluorescent probe for alkaline phosphatase with high sensitivity

Publication date: Available online 16 April 2019Source: Chinese Chemical LettersAuthor(s): Yang Yangyang, Zhang Chen, Pan Rizhao, Zhang Shiwei, Yao Shengtai, Tang Yao, Zhu Weilong, Wang Liyue, Zhu Weiping, Xu Yufang, Qian XuhongAbstractAlkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-hydroxyphenyl)benzamide (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL. The probe also shows excellent biocompatibility and has been applied for monitoring the endogenic ALP in living cells.Graphical abstrcatAlkaline phosphatase (ALP) is one of essential biomarkers in mammalian tissue. Here we report a ratiometric probe for ALP, which is rationally designed and synthesized by employing ESIPT fluorophore N-(3-(benzo[d]thiazol-2-yl)-4-(hydroxyphenyl) benzamide) (BTHPB). The enzymatic dephosphorylation converts the probe to BTHPB, which exhibits a large spectral red-shift (120 nm), allowing extremely high sensitivity of ALP sensing at 0.004 mU/mL.

Source: Chinese Chemical Letters - April 17, 2019 Category: Chemistry Source Type: research