Bub1--the zombie protein that CRISPR cannot kill

The conserved Bub1 kinase was proposed to be non-essential for the mitotic spindle assembly checkpoint based on recent findings that knocking-out Bub1 by CRISPR/Cas9 did not impair checkpoint function in human cells. New studies now demonstrate that CRISPR/Cas9 Bub1 knockout cells still express low levels of Bub1 protein and that only removal of this remaining Bub1 pool by RNA interference substantially weakens spindle assembly checkpoint signaling.

Source: EMBO Journal - March 31, 2019 Category: Molecular Biology Authors: Meraldi, P. Tags: Cell Cycle, Chromatin, Epigenetics, Genomics & Functional Genomics, Methods & Resources News [amp ] Views Source Type: research