Investigation of direct counting and sizing of DNA fragments in flow applying an improved data analysis and correction method

In this study, we analysed raw detector signals and the sizing performance for target identification and the effect of coincidence detection concerning concentration measurements. We present data of purified artificial DNA samples measured with the home-built setup. Main emphasis was to develop an improved data analysis method to gain insight into and carefully correct for coincident detection of DNA fragments and for estimation of the amount of fragment dimers.
Source: Biomolecular Detection and Quantification - Category: Molecular Biology Source Type: research