Development and characterization of a canine-specific anti-CD94 (KLRD-1) monoclonal antibody

Publication date: Available online 14 March 2019Source: Veterinary Immunology and ImmunopathologyAuthor(s): Scott S. Graves, Boglarka Gyurkocza, Diane M. Stone, Maura H. Parker, Kraig Abrams, Christoph Jochum, Susanna Gallo, Marium Saad, Melissa M. Johnson, Steven L. Rosinski, Rainer StorbABSTRACTNatural killer (NK) cells are non-T, non-B lymphocytes are part of the innate immune system and function without prior activation. The human NK cell surface determinant, CD94, plays a critical role in regulation of NK cell activity as a heterodimer with NKG2 subclasses. Canine NK cells are not as well defined as the human and murine equivalents, due in part to the paucity of reagents specific to cell surface markers. Canines possess NK/NKT cells that have similar morphological characteristics to those found in humans, yet little is known about their functional characteristics nor of cell surface expression of CD94. Here, we describe the development and function of a monoclonal antibody (mAb) to canine (ca) CD94. Freshly isolated canine CD94+ cells were CD3+/–, CD8+/–, CD4–, CD21–, CD5low, NKp46+, and were cytotoxic against a canine target cell line. Anti-caCD94 mAb proved useful in enriching NK/NKT cells from PBMC for expansion on CTAC feeder cells in the presence of IL-2 and IL-15. The cultured cells were highly cytolytic with co-expression of NKp46 and reduced expression of CD3. Transmission electron microscopy revealed expanded CD94+ lymphocytes were morphologically large ...
Source: Veterinary Immunology and Immunopathology - Category: Veterinary Research Source Type: research