Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation.

Protein aggregation capture on microparticles enables multi-purpose proteomics sample preparation.
Mol Cell Proteomics. 2019 Mar 04;:
Authors: Batth TS, Tollenaere MAX, Rüther PL, Gonzalez-Franquesa A, Prabhakar BS, Bekker-Jensen SH, Deshmukh AS, Olsen JV
Abstract
Universal proteomics sample preparation is challenging due to the high heterogeneity of biological samples. Here we describe a novel mechanism that exploits the inherent instability of denatured proteins for non-specific immobilization on microparticles by protein aggregation capture. To demonstrate the general applicability of this mechanism, we analyzed phosphoproteomes, tissue proteomes, and interaction proteomes as well as dilute secretomes. The findings presents a practical, sensitive and cost-effective proteomics sample preparation method.
PMID: 30833379 [PubMed - as supplied by publisher]

Source: Molecular and Cellular Proteomics : MCP - March 3, 2019 Category: Molecular Biology Authors: Batth TS, Tollenaere MAX, Rüther PL, Gonzalez-Franquesa A, Prabhakar BS, Bekker-Jensen SH, Deshmukh AS, Olsen JV Tags: Mol Cell Proteomics Source Type: research