Values of diagnostic tests for the various species of spirochetes.

Values of diagnostic tests for the various species of spirochetes. Med Mal Infect. 2019 Feb 11;: Authors: Eldin C, Jaulhac B, Mediannikov O, Arzouni JP, Raoult D Abstract Bacteria of the Borrelia burgdorferi sensu lato complex, responsible for Lyme disease, are members of the spirochetes phylum. Diagnostic difficulties of Lyme disease are partly due to the characteristics of spirochetes as their culture is tedious or even impossible for some of them. We performed a literature review to assess the value of the various diagnostic tests of spirochetes infections of medical interest such as Lyme borreliosis, relapsing fever borreliae, syphilis, and leptospirosis. We were able to draw similarities between these four infections. Real-time PCR now plays an important role in the direct diagnosis of these infections. However, direct diagnosis remains difficult because of a persistent lack of sensitivity. Serological testing is therefore crucial in the diagnostic process. All currently available diagnostic tools are imperfect, with a potential risk of false positive and false negative results depending on the clinical context. Physicians should always take into consideration the clinical and epidemiological context when Lyme disease, relapsing fever borreliae, syphilis, and leptospirosis are suspected. PMID: 30765286 [PubMed - as supplied by publisher]
Source: Medecine et Maladies Infectieuses - Category: Infectious Diseases Tags: Med Mal Infect Source Type: research

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We present a case of a man with genital condyloma acuminatum with a positive T. pallidum IHC stain but negative T. pallidum serologies and no syphilitic symptoms. It has been shown that the T. pallidum antibody IHC can cross-react, staining other spirochetes, including Borrelia burgdorferi and the Brachyspira family of intestinal spirochetes. Because of the proximity of our patient's lesions to the anus, and the persistently negative T. pallidum serologies, we believe nontreponemal spirochetes colonized the condyloma, giving a false-positive T. pallidum IHC. This cross-reactivity is a potential diagnostic pitfall and is im...
Source: The American Journal of Dermatopathology - Category: Pathology Tags: Brief Report Source Type: research
We describe a 20-year-old woman with left seventh nerve palsy secondary to latent syphilis of unknown duration, with complete recovery after penicillin G benzathine. In addition to reactive rapid plasma reagin and positive T. pallidum particle agglutination testing, Lyme enzyme-linked immunosorbent assay was equivocal, and Lyme Western blot was positive for immunoglobulin M. In syphilis, Lyme enzyme-linked immunosorbent assay may be falsely positive, but Western blot is known to remain negative. Similarly, in LD, fluorescent treponemal antibody absorption testing may be falsely positive, but rapid plasma reagin remains non...
Source: Infectious Diseases in Clinical Practice - Category: Infectious Diseases Tags: Case Reports Source Type: research
ConclusionsOur results show that Tp0136 facilitates differential level of binding to cell lines representing various host tissues, which highlights the importance of this protein in colonization of human organs byT.pallidum and resulting syphilis pathogenesis.
Source: PLoS Neglected Tropical Diseases - Category: Tropical Medicine Authors: Source Type: research
CONCLUSIONS: Immunization with native antigens might not be sufficient to obtain complete protection to infection. Nonetheless we showed that non-infectious B. burgdorferi can be an effective carrier to deliver and elicit a specific host response to T. pallidum antigens to assess the efficacy of syphilis vaccine candidates. PMID: 30797635 [PubMed - as supplied by publisher]
Source: Vaccine - Category: Allergy & Immunology Authors: Tags: Vaccine Source Type: research
The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme immunoassays (EIAs) (TestLine), Liaison chemiluminescence (DiaSorin), ELISA-Viditest (Vidia), EIA, and Blot-Line (TestLine) using recombinant antigens. All tests were performed on a panel of 90 samples from patients with clinically characterized borreliosis (53 w...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
Conclusions:By education and restricted use initiatives, inappropriate CSF Lyme PCR testing for diagnosing neuroborreliosis was significantly affected. This initiative led to reduced healthcare costs for the institution and patient while improving evidence based medical practices.Disclosure: Dr. Vanino has nothing to disclose. Dr. Baldwin has nothing to disclose.
Source: Neurology - Category: Neurology Authors: Tags: Infectious Disease: HIV, Syphilis, Borrelia, TB, Cysticercosis, and Other Infections Source Type: research
Conclusions:With this review we intend to demonstrate the wide range of neurological symptoms that LN can cause, and enhance that epidemiological context and clinical suspicion are important factors to the diagnosis.Disclosure: Dr. Espírito Santo has nothing to disclose. Dr. Almendra has nothing to disclose. Dr. Mendes has nothing to disclose. Dr. Veiga has nothing to disclose. Dr. Costa has nothing to disclose. Dr. Velon has nothing to disclose. Dr. Guimarães has nothing to disclose.
Source: Neurology - Category: Neurology Authors: Tags: Autoimmunity with Infection, Syphilis, Lyme, Tuberculosis, and other Bacteria Source Type: research
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Source: Neurology - Category: Neurology Authors: Tags: Autoimmunity with Infection, Syphilis, Lyme, Tuberculosis, and other Bacteria Source Type: research
This article is protected by copyright. All rights reserved. Swimming with sugar: We demonstrate that the flagellar filament proteins of oral bacterium Treponema denticola are modified with a novel glycan (a C7‐acylated pseudaminic acid derivative, m/z 451.2) and that such a modification is essential for flagellar assembly and motility.
Source: Molecular Microbiology - Category: Microbiology Authors: Tags: Research Article Source Type: research
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Source: Healthy Living - The Huffington Post - Category: Consumer Health News Source Type: news
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