HPLC-PDA analysis of ACE-inhibitors, hydrochlorthiazide and indapamide utilizing Design of Experiments

Publication date: Available online 18 November 2014 Source:Arabian Journal of Chemistry Author(s): Eyad Rashed Dawud , Ashok K. Shakya A simple, rapid, precise, accurate and sensitive high performance liquid chromatographic method has been developed for simultaneous determination of ACE inhibitors with hydrochlorothiazide and indapamide in pharmaceutical formulations. ‘Design of Experiments’ (DoE) using ‘central composite design’ (CCD) was applied to facilitate method development and optimization. Mobile phase was optimized utilizing response surface methodology using Design Expert software. Chromatographic separation was achieved on Hypersil®-Gold C18 (100 x 4.6 mm, 3 μm, Thermo Fisher Scientific, USA), column at 25 °C. The mobile phase was 58% Buffer (5 mM KH2PO4, containing triethylamine 0.25 ml/L), 25% acetonitrile and 17% methanol (pH adjusted to 2.8±0.1). The analysis was performed at 215 nm. The mobile phase flow rate was 1.0 ml/min and injection volume 10 μl. The method was validated for linearity, limits of quantitation and detection, accuracy, precision, ruggedness and robustness as per the International Conference on Harmonization (ICH) guidelines. Calibrations curves (for lisinopril, hydrochlorothiazide, captopril, imidapril, perindopril, indapamide and trandolapril) were linear in the concentration range of 5-35 μg/ml. The limit of detection and limit of quantitation for experimental drugs were ranged from 0.03 to 0.61 and 0.08 to 1.84 μg/ml ...
Source: Arabian Journal of Chemistry - Category: Chemistry Source Type: research