Mcm10 has potent strand-annealing activity and limits translocase-mediated fork regression [Chemistry]

The 11-subunit eukaryotic replicative helicase CMG (Cdc45, Mcm2-7, GINS) tightly binds Mcm10, an essential replication protein in all eukaryotes. Here we show that Mcm10 has a potent strand-annealing activity both alone and in complex with CMG. CMG-Mcm10 unwinds and then reanneals single strands soon after they have been unwound in...

Source: Proceedings of the National Academy of Sciences - January 15, 2019 Category: Science Authors: Ryan Mayle, Lance Langston, Kelly R. Molloy, Dan Zhang, Brian T. Chait, Michael E. O’Donnell Tags: Biological Sciences Source Type: research