Qualitative analysis of the roots of salvia miltiorrhiza and Salvia yunnanensis based on NIR, UHPLC and LC-MS-MS

Publication date: Available online 9 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Lijun Ni, Fangfang Zhang, Mingyue Han, Liguo Zhang, Shaorong Luan, Wei Li, Haixing Deng, Zhuhui Lan, Zhongxiang Wu, Xiaofang Luo, Leslaw MleczkoAbstractNear infrared spectroscopy (NIR) was applied to discriminate the roots of salvia miltiorhiza Bunge (Danshen for short) and salvia yunnanensis C. H. Wright (Zidanshen for short) by means of principal component analysis (PCA), improved and simplified K nearest neighbors (IS-KNN). Furthermore, an ultra-high performance liquid chromatographic (UHPLC) coupled with photodiode-array detector was developed for building fingerprints of lipophilic components of Danshen and Zidanshen, respectively. Basing on NIR information, both PCA and IS-KNN method classified the two kinds of Chinese medical herbs with 100% accuracy. The chromatographic fingerprints of the lipophilic components of Danshen and Zidanshen have 10 and 12 common peaks, respectively. Liquid chromatography coupled with mass spectroscopy (LC-MS-MS) was applied to identify these peaks. Among these, three small peaks in the fingerprints of Zidanshen are not found in Danshen, one of which was identified as α-lapachone, and the other two compounds were not yet identified; a small peak after tanshinone IIA in the fingerprints of Danshen was not found in Zidanshen, which was identified as miltirone. The two herbs have 10 common lipophilic components. The similarity ...

Source: Journal of Pharmaceutical and Biomedical Analysis - January 10, 2019 Category: Drugs & Pharmacology Source Type: research