Site-specific functionalization of proteins and their applications to therapeutic antibodies

Publication date: February 2014 Source:Computational and Structural Biotechnology Journal, Volume 9, Issue 14 Author(s): Remko van Vught , Roland J Pieters , Eefjan Breukink Protein modifications are often required to study structure and function relationships. Instead of the random labeling of lysine residues, methods have been developed to (sequence) specific label proteins. Next to chemical modifications, tools to integrate new chemical groups for bioorthogonal reactions have been applied. Alternatively, proteins can also be selectively modified by enzymes. Herein we review the methods available for site-specific modification of proteins and their applications for therapeutic antibodies.
Source: Computational and Structural Biotechnology Journal - Category: Biotechnology Source Type: research