Biosensors for measuring matrix metalloproteinases: an emerging research field

Publication date: Available online 3 November 2018Source: TrAC Trends in Analytical ChemistryAuthor(s): Arno Kirchhain, Noemi Poma, Pietro Salvo, Lorena Tedeschi, Bernardo Melai, Federico Vivaldi, Andrea Bonini, Maria Franzini, Laura Caponi, Arianna Tavanti, Fabio Di FrancescoAbstractMatrix metalloproteinases (MMPs) have been proposed as markers of many pathological conditions for their ability to degrade extracellular matrix components and remodel tissues. This review presents an overview of biosensors for the measurement of MMPs in serum, sputum and cell cultures. Major benefits and limitations of these technologies are discussed with respect to zymography and immunoassays, which are the reference methods to measure MMP activity and concentration. The paper also compares the analytical performances of sensors to the specific requirements for the application in clinical research, and comments on future trends in this field.

Source: TrAC Trends in Analytical Chemistry - November 3, 2018 Category: Chemistry Source Type: research