Crystallization and preliminary X-ray analysis of the hypothetical deaminase RPB_0146 from Rhodopseudomonas palustris HaA2

RPB_0146, a putative deaminase from Rhodopseudomonas palustris HaA2, was expressed in Escherichia coli BL21 (DE3) cells and purified using a His6 tag by Ni2+-chelating affinity chromatography for X-ray crystallographic analysis. Diffraction-quality crystals were grown by the hanging-drop vapour-diffusion method at 289 K and diffracted to a resolution of 2.44 Å using a wavelength of 1.000 Å at the Photon Factory (KEK), Japan. The crystals belonged to the orthorhombic space group P212121, with unit-cell parameters a = 66.26, b = 123.94, c = 155.95 Å.

Source: Acta Crystallographica Section F - October 31, 2014 Category: Biochemistry Authors: Zhang, G.Yu, D.Yang, G.Dong, H.Zhang, T.Liu, X. Tags: RPB_0146 deaminase Rhodopseudomonas palustris HaA2 crystallization communications Source Type: research