An Innovative Multiplexed And Flexible Molecular Approach For The Differential Detection Of Arboviruses

Publication date: Available online 28 September 2018Source: The Journal of Molecular DiagnosticsAuthor(s): Fanny Leon, Albert Meyer, Robin Reynier, Emilie Blanc, Lilian Bruyère-Ostells, Jean-Charles Brès, Yannick Simonin, Sara Salinas, Pierre Gallian, Isabelle Leparc-Goffart, Antoine Biron, Myrielle Dupont-Rouzeyrol, François Morvan, Jean-Jacques Vasseur, Vincent Foulongne, Philippe Van de Perre, Jean-François Cantaloube, Chantal Fournier-WirthAbstractNucleic acid testing during the preseroconversion viremic phase is required to differentially diagnose arboviral infections. The continuing emergence of arboviruses such as Zika (ZIKV), dengue (DENV), and chikungunya (CHIKV) viruses necessitates the development of a flexible diagnostic approach. Similar clinical signs and the priority to protect pregnant women from ZIKV infection indicate that the differential diagnosis of arboviruses is essential for effective patient management, clinical care, and epidemiological surveillance. Here, we describe an innovative diagnostic approach combining generic reverse transcription-PCR amplification and identification by hybridization to specific probes. Original tetrathiolated probes were designed for the robust, sensitive, and specific detection of amplified arboviral genomes. The limit of detection using cultured and quantified stocks of whole viruses was 1 TCID50/mL for DENV-1, DENV-3, and CHIKV and 10 TCID50/mL for DENV-2, DENV-4, and ZIKV. The assay showed 100% specificity with no ...
Source: The Journal of Molecular Diagnostics - Category: Pathology Source Type: research