GSE104582 Transcriptome and metabolome analysis support that nitrate primarily promotes N and C metabolism in soybean roots and reduces those in nodules.

Contributors : Takuji Ohyama ; Shinji Ishikawa ; Yuki Ono ; Norikuni Ohtake ; Kuni SueyoshiSeries Type : Expression profiling by arrayOrganism : Glycine maxTo dissect differences in gene expression profile of soybean roots and root nodules, we have employed microarray analysis. Seeds of soybean (Glycine max L. cv. Nourin No. 2) were inoculated with rhizobia (Bradyrhizobium diazoefficiens USDA110) and were hydroponically cultivated under controlled conditions with nitrogen free culture solution (Saito et al. 2014). At 19 days after planting, each plant were treated with or without 5 mM nitrate for 24 hours. Roots and nodules from three plants were pooled with three biological replications, and total RNA was extracted.

Source: GEO: Gene Expression Omnibus - October 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Glycine max Source Type: research