GSE107721 BART-seq: cost-effective massively parallel targeted sequencing for genomics and transcriptomics [transcriptomics]

We report the application of a novel workflow, BART-Seq (Barcode Assembly foR Targeted Sequencing), the first highly sensitive, quantitative, and inexpensive technique for enriching selected cohorts of transcripts and genomic regions from thousands of samples in parallel, for next-generation sequencing (NGS). Multiplexing is based on a simple method for producing virtually unlimited matrices of barcoded primer sets. The method provides sample-specific amplicon labels for hundreds-fold enrichment compared to unbiased transcriptomics and genomics approaches. We demonstrated the technique by quantitative analysis of pluripotency genes in bulk cell preparations and thousands of single cells. We believe that BART-Seq will complement low-sensitivity global NGS approaches, such as droplet-based sequencing, by enabling analysis, validation, and screening of specific processes in biomedical research.

Source: GEO: Gene Expression Omnibus - October 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Other Homo sapiens Source Type: research