α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster.

α-actinin accounts for the bioactivity of actin preparations in inducing STAT target genes in Drosophila melanogaster. Elife. 2018 Sep 27;7: Authors: Gordon O, Henry CM, Srinivasan N, Ahrens S, Franz A, Deddouche S, Chakravarty P, Phillips D, George R, Kjaer S, Frith D, Snijders AP, Valente RS, Simoes da Silva CJ, Teixeira L, Thompson B, Dionne MS, Wood W, Reis E Sousa C Abstract Damage-associated molecular patterns (DAMPs) are molecules exposed or released by dead cells that trigger or modulate immunity and tissue repair. In vertebrates, the cytoskeletal component F-actin is a DAMP specifically recognised by DNGR-1, an innate immune receptor. Previously we suggested that actin is also a DAMP in Drosophila melanogaster by inducing STAT-dependent (Srinivasan et al., 2016). Here, we revise that conclusion and report that α-actinin is far more potent than actin at inducing the same STAT response and can be found in trace amounts in actin preparations. Recombinant expression of actin or α-actinin in bacteria demonstrated that only α-actinin could drive the expression of STAT target genes in Drosophila. The response to injected α-actinin required the same signalling cascade that we had identified in our previous work using actin preparations. Taken together, these data indicate that α-actinin rather than actin drives STAT activation when injected into Drosophila. PMID: 30260317 [PubMed - as supplied by publisher]
Source: Genomics Proteomics ... - Category: Genetics & Stem Cells Authors: Tags: Elife Source Type: research