The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels.

In this study, we have developed a computational pipeline for designing the oligonucleotides capable of covering a significant number of known viruses within various taxonomic orders, as well as their novel variants. We have subsequently designed a genus-specific oligonucleotide panel for targeted enrichment of viral nucleic acids in biological material and demonstrated the possibility of its application for virus detection in bird samples. We have tested our panel using a number of collected samples and have observed superior efficiency in the detection and identification of viral pathogens. Since a reliable, bioinformatics-based analytical method for the rapid identification of the sequences was crucial, an NGS-based data analysis module was developed in this study, and its functionality in the detection of novel viruses and analysis of virome diversity was demonstrated.
PMID: 30158979 [PubMed]

Source: Adv Data - September 1, 2018 Category: Epidemiology Authors: Ayginin AA, Pimkina EV, Matsvay AD, Speranskaya AS, Safonova MV, Blinova EA, Artyushin IV, Dedkov VG, Shipulin GA, Khafizov K Tags: Adv Virol Source Type: research