Differential diagnosis of fowlpox and infectious laryngotracheitis viruses in chicken diphtheritic manifestations by mono and duplex real-time PCR.

Differential diagnosis of fowlpox and infectious laryngotracheitis viruses in chicken diphtheritic manifestations by mono and duplex real-time PCR.
Avian Pathol. 2014 Oct 15;:1-13
Authors: Irit D, Raibstein I, Altory A
Abstract
Infectious laryngotracheitis (ILTV) and fowlpox (FPV) viruses cause diphtheritic lesion in chicken tracheas and can simultaneously infect the same bird. A differential molecular diagnostic test, the duplex real-time PCR is now reported using ILTV and FPV vaccine viruses and clinical samples from chickens, either uninfected, or naturally-infected with ILTV, or FPV, or with both viruses. The dual virus amplification by real-time PCR was demonstrated to behave similarly to monoplex amplification, in spite of the fact that the real time exponential amplification plots of the vaccine viruses were more illustrative than those of the clinical samples.
PMID: 25317604 [PubMed - as supplied by publisher]

Source: Avian Pathology - October 15, 2014 Category: Pathology Authors: Irit D, Raibstein I, Altory A Tags: Avian Pathol Source Type: research