Pranoprofen quantification in ex vivo corneal and scleral permeation samples: Analytical validation

Publication date: Available online 18 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): C. Cañadas-Enrich, G. Abrego, H.L. Alvarado, A.C. Calpena-Capmany, A. Boix-MontañesAbstractThe investigation of the ocular permeability and/or distribution of pranoprofen (PF), a non-steroidal antiinflamatory drug, demands for the selective analysis of its transit through specific ocular membranes. Therefore, customised ex vivo permeation experiments through external ocular tissues (cornea and sclera) have been validated for this drug in addition to its HPLC-UV quantification following standard bioanalytical guidelines. Chromatographic conditions consist of an isocratic system to elute the drug with a C18 column with UV detection at 245 nm. Precision, expressed as the relative standard deviation (% RSD), ranged between 4.89 and 0.79% (intra-day) and between 9.02 and 2.14% (interday). Accuracy ranged between 5.15 and -1.92% in intra-day experiments and between 6.25 and −4.89% in inter-day experiments. Drug recovery from tissue samples was reproducible around 90% and considered satisfactory to adequately assess drug levels in target tissues. Results indicate that the procedure is valid for the quantitation of PF in those ophthalmic samples in the range of 6.5 μg/mL to 100 μg/ml. As a proof of concept, PF permeation profiles through porcine cornea and sclera with vertical diffusion cells have been generated and analyzed. Pilot experim...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Publication date: Available online 10 November 2019Source: Urology Case ReportsAuthor(s): Jonathan Kopel, Pranav Sharma, Irfan WarriachAbstractSolitary fibrous tumors (SFTs) are rare mesenchymal neoplasms found areas lining the pleura. First reported in 1931, there have been a total of 55 cases of SFTs of the kidney worldwide. In most cases, SFTs presents with hematuria, flank pain, and enlarging abdominal mass. In this case, we report a case of a patient who underwent a radical nephrectomy to remove an SFTs of the kidney with compression of the vena cava. This case further expands upon the importance of diagnosing and ass...
Source: Urology Case Reports - Category: Urology & Nephrology Source Type: research
AbstractHyperlipidemia and oxidative stress are indispensable features of chronic kidney disease (CKD) that favor the development of atherogenic plaques and cardiovascular disease (CVD). A number of vasoactive mediators including proprotein convertase subtilisin –kexin type 9 (PCSK9), endothelin-1, nitric oxide, and angiotensin II have fundamental roles in the pathophysiology of atherosclerotic events; moreover, their levels are affected by dyslipidemia and oxidative stress due to renal dysfunction. Therefore, therapeutic measures aimed at correcting dysl ipidemia and alleviating oxidative stress could potentially pr...
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
AbstractThe prevalence of asymptomatic HUA is increasing year after year. HUA is a risk factor for the occurrence and development of renal diseases. However, the role of urate-lowering therapy in asymptomatic HUA complicated by renal damage is still controversial. In some experiments, the treatment of asymptomatic HUA complicated by renal damage may delay the progression of kidney damage. In addition, there is increasing evidence, suggesting that elevated serum uric acid is an independent risk factor for kidney disease. However, in other studies, uric acid-lowering therapy did not improve renal function, and uric acid leve...
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
ConclusionsTrimetazidine did not exert significant renal protective effect on preventing CIN and in hosptial  major adverse clinical events in unselected patients undergoing PCI.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
ConclusionsUnmarried men are at higher risk of not benefiting of cytoreductive nephrectomy, metastasectomy, or systemic therapy than their married counterparts. Unmarried women are at higher risk of not benefiting of cytoreductive nephrectomy or systemic therapy. These gender-related differences cumulate in higher CSM in unmarried men, but not in unmarried women.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
We examined the relationship between baseline MetS and renal dysfunction after a 3-year evaluation based on estimated glomerul ar filtration rate (eGFRCKDEPI) using the CKD-EPI equations modified by the Japan coefficient. CKD was defined as dipstick-positive proteinuria (>  or = 1 +) or a low eGFRCKDEPI (
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
ConclusionsFebuxostat presents a superior effect in delaying renal impairment progression compared with allopurinol in CKD patients complicated with HUA.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
AbstractKidney injury molecule-1 (KIM-1) staining has been shown to be very useful in identifying acute proximal tubular injury, but its sensitivity, specificity and predicting values for the recovery of renal function after injury in renal biopsies have not been well established. In the first study, we randomly selected 184 renal biopsies from a wide age range of patients (children to elderly) with various renal diseases. KIM-1 staining scores were significantly correlated with serum creatinine (sCr) levels (P 
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
ConclusionIn renal cell carcinoma, rapid and successful drug development has resulted in multiple treatment options, requiring careful decision making for individual patients and have emphasized how newly developed therapies work.
Source: International Urology and Nephrology - Category: Urology & Nephrology Source Type: research
Marc Rodwin of Suffolk University Law School reports that the French health system controls drug prices by determining maximum prices based on added therapeutic value and external reference pricing; employing negotiation to set prices and limit price increases; and capping total spending to a global budget.        
Source: The Commonwealth Fund: Publications - Category: International Medicine & Public Health Authors: Source Type: research
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