A fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis.

A fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis.
Angew Chem Int Ed Engl. 2018 Jul 10;:
Authors: Kuijpers K, Bottecchia C, Cambie D, Drummen K, Koenig N, Noel T
Abstract
Herein, we report the first fully automated continuous-flow platform for fluorescence quenching studies and Stern-Volmer analysis. All the components of the platform were automated and controlled by a self-written Python script. A user-friendly software allows even inexperienced operators to perform automated screening of novel quenchers or Stern-Volmer analysis, thus accelerating and facilitating both reaction discovery and mechanistic studies. The operational simplicity of our system affords a time and labor reduction over batch methods, while increasing the accuracy and reproducibility of the data produced. Finally, the applicability of our platform is elucidated through relevant case studies.
PMID: 29992682 [PubMed - as supplied by publisher]

Source: Angewandte Chemie - July 10, 2018 Category: Chemistry Authors: Kuijpers K, Bottecchia C, Cambie D, Drummen K, Koenig N, Noel T Tags: Angew Chem Int Ed Engl Source Type: research