Rapid detection of goose hemorrhagic polyomavirus using TaqMan quantitative real-time PCR

In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses. The standard curve had a linear correlation of 0.997 and efficiency of 99% between the cycle threshold value and the logarithm of the plasmids copy number. The possible lowest detectable concentration was 35.4 copies/μl; 100 times more sensitive than conventional PCR (detection limit, 3.54 × 103 copies/μl). Domestic Jinyun Sheldrakes ducks and their embryonated eggs were found positive of GHPV infection which provides evidence of possible vertical transmission of GHPV.

Source: Molecular and Cellular Probes - July 11, 2018 Category: Molecular Biology Source Type: research