GSE112622 Species-specific endogenous  retroviruses drive widespread, heritable changes in the oocyte methylome

Contributors : Julie Brind'Amour ; Hisato Kobayashi ; Julien R Albert ; Kenjiro Shirane ; Akihiko Sakashita ; Aaron B Bogutz ; Tasuku Koike ; Mohammad M Karimi ; Louis L Lefebvre ; Tomohiro Kono ; Matthew C LorinczSeries Type : Expression profiling by high throughput sequencing ; Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculus ; Rattus norvegicusDe novo  DNA methylation (DNAme) occurs coincident with transcription during mouse oogenesis. As many oocyte transcripts originate in Long Terminal Repeats (LTRs), which are divergent across species, we examined whether polymorphic LTR-initiated transcription units (LITs) shape the oocyte methylome. We ide ntified thousands of syntenic regions in mouse, rat and human, including CpG islands (CGIs), that show divergent DNAme associated with polymorphic LITs. Notably, many CGI promoters methylated exclusively in mouse and/or rat are embedded within rodent-specific LITs, and show persistent maternal me thylation in the blastocyst. Polymorphic LITs are also responsible for divergent methylation of CGI promoters in distantly related mouse strains, revealing that LITs also promote intra-species diversification of promoter DNAme.

Source: GEO: Gene Expression Omnibus - July 6, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Mus musculus Rattus norvegicus Source Type: research