GSE99805 The Nonstop decay and the RNA silencing systems operate cooperatively in plants

Contributors : Istv án Szádeczky-Kardoss ; Tibor Csorba ; Andor Auber ; Anita Schamberger ; Tünde Nyikó ; János Taller ; Tamás I Orbán ; József Burgyán ; Dániel SilhavySeries Type : Expression profiling by high throughput sequencingOrganism : Arabidopsis thaliana ; Nicotiana benthamianaTranslation-dependent mRNA quality control systems protect the protein homeostasis of eukaryotic cells by eliminating aberrant transcripts and stimulating the decay of their protein products. Although these systems are intensively studied in animals, little is known about the translation-dependent quality control systems in plants. Here we characterize the mechanism of nonstop decay (NSD) system in Nicotiana benthamiana model plant. We show that plant NSD efficiently degrades nonstop mRNAs, which can be generated by premature polyadenylation, and stop codon-less transcripts, which can be produced by endonucleolytic cleavage. We demonstrate that in plants, like in animals, Pelota, Hbs1 and SKI2 proteins are required for NSD, supporting that NSD is an ancient and conserved eukaryotic quality control system. Relevantly, we found that NSD and RNA silencing systems cooperate in plants. Plant silencing predominantly represses target mRNAs through endonucleolytic cleavage in the coding region. The cleavage products are degraded by general decay systems or subjected to silencing amplification. The balance between decay and silencing amplification might be finely regulated by unknown m...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Arabidopsis thaliana Nicotiana benthamiana Source Type: research