The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import.

The transmembrane nucleoporin Pom121 ensures efficient HIV-1 pre-integration complex nuclear import.
Virology. 2018 Jun 25;521:169-174
Authors: Guo J, Liu X, Wu C, Hu J, Peng K, Wu L, Xiong S, Dong C
Abstract
HIV-1 hijacks host classical cargo nuclear transportation, or nonclassical pathways by directly interacting with importin-β family proteins or nucleoporins for efficient pre-integration complex (PIC) nuclear import. Recently, an N-terminal truncated form of nucleoporin Pom121c (601-987 aa) was reported to inhibit HIV-1 replication. In contrast, we found that HIV-1 replication was significantly decreased in 293T and TZM-b1 cells with siRNA-mediated Pom121 knockdown. Quantitative PCR indicated that viral replication was impaired at the step of cDNA nuclear import. Furthermore, we found that karyopherin-β1 (KPNB1), which belongs to the importin-β family, interacts with Pom121 and is involved in Pom121-mediated PIC nuclear import. Rescue experiment indicated that the FG-repeats and the following α-helix in Pom121 are required for its role in HIV-1 PIC nuclear import. Taken together, our results showed that full-length Pom121 enables efficient PIC nuclear import, and suggested that this process may rely on KPNB1 dependent classical cargo nuclear transportation way.
PMID: 29957337 [PubMed - as supplied by publisher]

Source: Virology - June 25, 2018 Category: Virology Authors: Guo J, Liu X, Wu C, Hu J, Peng K, Wu L, Xiong S, Dong C Tags: Virology Source Type: research