Generation and Use of Site-Directed Chromosomal cyaA′ Translational Fusions in Salmonella enterica

CyaA from Bordetella pertussis is a calmodulin-dependent adenylate cyclase. Fusions to the catalytic domain of CyaA (CyaA′) are useful tools to detect translocation of type III secretion system effectors from gram-negative pathogens like Salmonella enterica. These fusions are usually generated using plasmids with strong promoters. Here, we describe a protocol to insert the CyaA′-encoding sequence in a specific site in the bacterial chromosome in order to get a monocopy fusion whose expression is driven by the native promoter. We also describe the procedure to detect translocation of a CyaA′ fusion into mammalian cells.
Source: Springer protocols feed by Infectious Diseases - Category: Infectious Diseases Source Type: news

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Publication date: Available online 30 May 2020Source: Journal of Hazardous MaterialsAuthor(s): Jun Ge, Haixin Wang, Jiayu Lin, Shengke Tian, Jianqi Zhao, Xianyong Lin, Lingli Lu
Source: Journal of Hazardous Materials - Category: Environmental Health Source Type: research
Neuroprotective effect of tormentic acid against memory impairment and neuro‑inflammation in an Alzheimer's disease mouse model. Mol Med Rep. 2020 May 18;: Authors: Cui W, Sun C, Ma Y, Wang S, Wang X, Zhang Y Abstract Cognitive impairment and neuro‑inflammatory responses are the distinctive characteristics of Alzheimer's disease (AD). Tormentic acid (TA) is one of the major active components of Potentilla chinensis and has been demonstrated to have anti‑inflammatory properties. However, the potential effects of TA on neuro‑inflammatory responses and memory impairment in AD remain unknown. ...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research
Conclusions: This study provides novel insights into the transcriptomic remodelling that occurs in C. goreaui during transition to a symbiotic lifestyle, with important implications for understanding the establishment of symbiosis between corals and their dinoflagellate partners.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Third-party reanalysis Cladocopium goreaui Cladocopium sp. C1 Source Type: research
Contributors : Grihalakshmi Kakani ; Palmy R Jesudhasan ; Suresh D PillaiSeries Type : Expression profiling by arrayOrganism : Salmonella enterica subsp. enterica serovar TyphimuriumThe oxidative stress response to chlorine was analysed by the global gene expression profile for Salmonella Typhimurium
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Salmonella enterica subsp. enterica serovar Typhimurium Source Type: research
AbstractWe herein describe soft tissue tumor arising in the lower extremity of a pediatric patient. The tumor displayed a unique and wide range of histological features, sheet-like and cohesive growth pattern consisting of enlarged round to epithelioid atypical cells with a large alveolar and pseudopapillary histological architecture, focally mimicking alveolar soft part sarcoma and MiT family translocation renal cell carcinoma. Tumor cells were focally immunoreactive for cytokeratin, S-100, and EMA. RNA sequencing identified a novel in-frameNR1D1 (exon 5)-MAML1 (exon 2) gene rearrangement resulting in the formation of a p...
Source: Virchows Archiv - Category: Pathology Source Type: research
In conclusion, APT appears to favorably modulate anti-inflammatory effects in chondrocytes making it a promising compound for OA treatment.Graphical AbstractInhibitory effects of Alpinetin on TNF- α-induced NF-κB activation resulted in destruction of cartilage in rat OA chondrocytesin vitro. The TNF- α receptor were stimulated by TNF-α, activating the cytoplasmic IκBα kinases(IKKS), then IKKs will be phosphorylated, and subsequently degraded by the ubiquitin-proteasome system. NF-κB transfer to the nucleus and bind various NF-κB regulates the NF-κB recognition site in t...
Source: Inflammation - Category: Allergy & Immunology Source Type: research
Abstract Zeranol is an approved but controversial growth-promoting agent for livestock in North America. It is a mycotoxin metabolite secreted by the Fusarium family fungi. The regulatory bodies in this region have established the acceptable daily intake and exposure below the level would not significantly increase the health risk for humans. However, their European counterparts have yet to establish an acceptable level and do not permit the use of this agent in farm animals. Given the growth-promoting ability of zeranol, its effect on energy metabolism was investigated in the current study. Our results indicated ...
Source: Chemosphere - Category: Chemistry Authors: Tags: Chemosphere Source Type: research
Publication date: June 2020Source: Journal of Global Antimicrobial Resistance, Volume 21Author(s): Shengzhi Yang, Wenwen Deng, Shuliang Liu, Xiumei Yu, Ghulam Raza Mustafa, Shujuan Chen, Li He, Xiaolin Ao, Yong Yang, Kang Zhou, Bei Li, Xinfeng Han, Xuebin Xu, Likou Zou
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
Abstract Typhoid fever is the result of a human host-restricted Salmonella enteric serotype typhi infection that causes enteric fever. Around 21 million people contract typhoid annually, with Pakistan's inhabitants at most risk amongst Asian countries where typhoid remains prevalent. Decades of indiscriminate antibiotic usage has driven the evolution of multidrug-resistant strains and more recently, extensively drug-resistant (XDR) strains of Salmonella enteric serotype typhi. Current reports of extensively drug-resistant typhoid fever outbreak in Pakistan are not only a major concern for Pakistan but also for hea...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
Isorhamnetin Induces Melanoma Cell Apoptosis via the PI3K/Akt and NF-κB Pathways. Biomed Res Int. 2020;2020:1057943 Authors: Duan R, Liang X, Chai B, Zhou Y, Du H, Suo Y, Chen Z, Li Q, Huang X Abstract Malignant melanoma is characterized by its bad prognosis for aggressiveness, drug resistance, and early metastasis. Isorhamnetin (3'-methoxy-3,4',5,7-tetrahydroxyflavone; IH) is a natural flavonoid that has been investigated for its antitumor effects in breast cancer, colon cancer, and gastric cancer through inducing cell apoptosis. Given its role in tumor inhibition, no research has been conducte...
Source: Biomed Res - Category: Research Authors: Tags: Biomed Res Int Source Type: research
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