GSE113248 A Muscle-Specific Enhancer RNA Mediates Cohesin Recruitment and Regulates Transcription in Trans

Contributors : Pei-Fang Tsai ; Stefania Dell ’Orso ; Joseph Rodriguez ; Karinna O Vivanco ; Kyung-Dae Ko ; Kan Jiang ; Aster H Juan ; Aishe A Sarshad ; Laura Vian ; Michelle Tran ; Dara Wang ; A H Wang ; Jelena Perovanovic ; Dimitros Anastasakis ; Evelyn Ralston ; Thomas Ried ; Hong-Wei Sun ; Markus Hafner ; Daniel R Larson ; Vit torio SartorelliSeries Type : Genome binding/occupancy profiling by high throughput sequencingOrganism : Mus musculusThe enhancer regions of the myogenic master regulator MyoD give rise to at least two enhancer RNAs. CEeRNA regulates transcription of the adjacent MyoD gene while DRReRNA affects expression of Myogenin in trans. We found that DRReRNA is recruited at the Myogenin locus where it colocalizes with Myogenin nascent transcripts. DRReRNA associates with the Cohesin complex and such association correlates with its transactivating properties. Despite being expressed in undifferentiated cells, Cohesin is not loaded on Myogenin until the cells start expressing DRReRNA, which is then required for Cohesin chromatin recruitment and maintenance. Functionally, depletion of either Cohesin or DRReRNA reduces chromatin accessibility, prevents Myogenin activation, and hinders muscle cell differentiation. Thus, DRReRNA ensures spatially appropriate Cohesin loading in trans to regulate gene expression.

Source: GEO: Gene Expression Omnibus - June 1, 2018 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Mus musculus Source Type: research